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Nck AntibodyRabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1527a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
Nck Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | P16333 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | Y531 |
| Calculated MW | 42864 Da |
| Gene ID 4690 | |
| Other Names NCK1, NCK, Cytoplasmic protein NCK1, SH2/SH3 adaptor protein NCK-alpha | |
| Target/Specificity A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen. | |
| Dilution WB~~1:500~2000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Nck Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
Nck Antibody - Protein Information | |
| Name NCK1 | |
| Synonyms NCK | |
| Function Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling | |
| Cellular Location Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments | |
Nck Antibody - Related products
AP8825a: NCK1 Antibody (N-term)
LY11471a: NCK1 Over-expression Lysate
BP8825a: NCK1 Antibody (N-term) Blocking Peptide
Nck Antibody - Application data
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A. Western blot analysis on Hela cell lysate using anti-Nck RabMAb (Cat. #AJ1527a) 1:10,000 dilution.
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Nck RabMAb (Cat. #AJ1527a).
Nck Antibody - Research Areas
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BACKGROUND
Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).
REFERENCES
1. W Li, P Hu, EY Skolnik, A Ullrich and J Schlessinge; Mol. Cell. Biol., 12 1992, 5824-5833, Vol 12, No. 12
2. M M Chou, J E Fajardo, and H Hanafusa; Mol Cell Biol. 1992 December; 12(12): 5834
