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Nck Antibody

Rabbit Monoclonal Antibody

  • WB - Nck Antibody AJ1527a
    A. Western blot analysis on Hela cell lysate using anti-Nck RabMAb (Cat. #AJ1527a) 1:10,000 dilution.
  • IHC - Nck Antibody AJ1527a
    B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-Nck RabMAb (Cat. #AJ1527a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P16333
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names Y531
Calculated MW 42864 Da
Gene ID 4690
Other Names Cytoplasmic protein NCK1, NCK adaptor protein 1, Nck-1, SH2/SH3 adaptor protein NCK-alpha, NCK1, NCK
Target/Specificity A synthetic peptide corresponding to residues close to the SH2 domain of human Nck was used as immunogen.
Dilution WB~~1:500~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNck Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCK1
Synonyms NCK
Function Adapter protein which associates with tyrosine- phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA. May play a role in cell adhesion and migration through interaction with ephrin receptors.
Cellular Location Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments
Research Areas
Citations (0)

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Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).


1. W Li, P Hu, EY Skolnik, A Ullrich and J Schlessinge; Mol. Cell. Biol., 12 1992, 5824-5833, Vol 12, No. 12
2. M M Chou, J E Fajardo, and H Hanafusa; Mol Cell Biol. 1992 December; 12(12): 5834

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Cat# AJ1527a
(40 western blots)
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