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NDUFAF1 Antibody

Rabbit Monoclonal Antibody

  • WB - NDUFAF1 Antibody AJ1528a
    A. Western blot analysis on (A) HeLa, (B) Jurkat and (C) human heart lysates using anti-NDUFAF1 RabMAb (Cat. #AJ1528a).
  • IHC - NDUFAF1 Antibody AJ1528a
    B. Immunohistochemical analysis of paraffin-embedded human liver using anti-NDUFAF1 RabMAb (Cat. #AJ1528a).
  • IF - NDUFAF1 Antibody AJ1528a
    C. Immunofluorescent staining of HeLa cells using anti-NDUFAF1 RabMAb (Cat. #AJ1528a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y375
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR2796
Calculated MW 37764 Da
Gene ID 51103
Other Names Complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1, NDUFAF1, CIA30
Target/Specificity A synthetic peptide corresponding to residues at the C terminus of human NDUFAF1 was used as an immunogen.
Dilution WB~~1:1000~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDUFAF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms CIA30
Function Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).
Cellular Location Mitochondrion.
Tissue Location Ubiquitous..
Research Areas
Citations (0)

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NDUFAF1 (CIA30) is a chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex 1) (1). Complex I is the largest multiprotein enzyme located in the inner mitochondrial membrane that catalyzes the transfer of electrons from NADH to coenzyme Q (CoQ). Little is known about the assembly process of the subunits into the mature complex, however, NDUFAF1 is one of two proteins found to be involved. NDUFAF1 expression is ubiquitous with a slightly higher expression in various heart tissues, kidney, lung and liver (2).


1. Vogel RO, et al. FEBS J. 272(20):5317-26, 2005
2. Janssen R., et al. Journal Human Genetics 110(3):264-270, 2002

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Cat# AJ1528a
(40 western blots)
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