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NEDD8 Antibody (N-term)

Rabbit Monoclonal Antibody

  • WB - NEDD8 Antibody (N-term) AJ1529a
    A. Western blot analysis on (A) Hela and (B) K562 cell lysate using anti-NEDD8 RabMAb (Cat. #AJ1529a), dilution 1:500. (C) Western blot analysis of immunoprecipitation pellet from HeLa cell lysate using anti-NEDD8 RabMAb. (Cat. #AJ1529a), dilution 1:500.
  • IHC - NEDD8 Antibody (N-term) AJ1529a
    B. Immunohistochemical analysis of paraffin-embedded human lung carcinoma using anti-NEDD-8 (N-term) RabMAb (Cat. #AJ1529a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15843
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names Y297
Calculated MW 9072 Da
Gene ID 4738
Other Names NEDD8, Neddylin, Neural precursor cell expressed developmentally down-regulated protein 8, NEDD-8, Ubiquitin-like protein Nedd8, NEDD8
Target/Specificity A synthetic peptide corresponding to residues in the N-term of human NEDD8 was used as immunogen. This antibody detects NEDD8 bound to cullins proteins (neddylated cullins) as well as free NEDD8.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEDD8 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEDD8
Function Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C- APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Cellular Location Nucleus. Note=Mainly nuclear
Tissue Location Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes
Research Areas
Citations (0)

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NEDD8 (Neural precursor cell-Expressed Developmentally Down-regulated genes) is an ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis (1). NEDD8 has been shown to conjugate to nuclear proteins in a manner analogous to ubiquination and sentrinization. The conjugation process is though to be catalyzed by thee enzymes; E1 (NEDD8-activation), E2 (NEDD8-conjugation) and E3 (NEDD8-ligation). NEDD8 is known to conjugate to Cul-4A and other cullins proteins via the carboxy-terminal Gly residue for ubiquitinylation (3) while NUB1 will negatively regulate its expression (4).


1. Kumar, S., et al. 1993. Biochem. Biophys. Res. Commun. 195: 393-399
2. Gong, L., et al. (1999) J. Biol. Chem. 274, 12036-12042
3. Kipreos, E.T., et al. 1996. Cell 85: 829-839
4. Katsumi Kito,et al. Biol. Chem., Vol. 276, Issue 23, 20603-20609, June 8, 2001

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Cat# AJ1529a
(40 western blots)
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