|Reactivity||Human, Mouse, Rat|
|Calculated MW||61517 Da|
|Other Names||Neurofilament light polypeptide, NF-L, 68 kDa neurofilament protein, Neurofilament triplet L protein, NEFL, NF68, NFL|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-term of human NF-L was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Neurofilament L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.|
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). NF-L self polymerizes and provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M (3). NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons (4).
1. Nixon, R.A., and Shea, T. B. (1992) Cell Motil. Cytoskeleton 22, 81-91
2. Julien, JP., Meyer, D., Flavell, D., Hurst, J., and Grosveld, F. (1986) Brain Res. 387, 243-250
3. Kim SK et al. J Neurosci Methods Dec 2006.
4. Lin H et al. Hum Mol Genet 14(23):3643-59, 2005.
If you have any additional inquiries please email technical services at email@example.com.