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Neurofilament L Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1531a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
Neurofilament L Antibody (C-term) - Product info | |
| Application | WB
|
| Primary Accession | P07196 |
| Reactivity | Human, Mouse, Rat |
| Clone Names | EP675Y |
| Calculated MW | 61517 Da |
| Gene ID 4747 | |
| Other Names NEFM, NEF3, NFM, Neurofilament medium polypeptide, Neurofilament triplet M protein;160 kDa neurofilament protein;Neurofilament 3 | |
| Target/Specificity A synthetic peptide corresponding to residues near the C-term of human NF-L was used as immunogen. | |
| Dilution WB~~1:1000 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions Neurofilament L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
Neurofilament L Antibody (C-term) - Protein Information | |
| Name NEFL | |
| Synonyms NF68, NFL | |
| Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber | |
Neurofilament L Antibody (C-term) - Related products
AP7332c: NEFL Antibody (Center R185)
LY11734a: NEFL Over-expression Lysate
Neurofilament L Antibody (C-term) - Application data
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A. Western blot analysis on SH-SY5Y lysate using anti-NF-L RabMAb (Cat. #AJ1531a), dilution 1:50,000
Neurofilament L Antibody (C-term) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). NF-L self polymerizes and provides a scaffold for NFs to form via cross-bridging with NF-H or NF-M (3). NF-L aggregation has been linked to the pathogenesis of neurodegenerative disease, such as degradation of motor neurons (4).
REFERENCES
1. Nixon, R.A., and Shea, T. B. (1992) Cell Motil. Cytoskeleton 22, 81-91
2. Julien, JP., Meyer, D., Flavell, D., Hurst, J., and Grosveld, F. (1986) Brain Res. 387, 243-250
3. Kim SK et al. J Neurosci Methods Dec 2006.
4. Lin H et al. Hum Mol Genet 14(23):3643-59, 2005.
