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Neurofilament M Antibody

Rabbit Monoclonal Antibody

     
  • WB - Neurofilament M Antibody AJ1532a
    Western blot analysis on (A) 293T and (B) SH-SY5Y cell lysates using anti-Neurofilament M RabMAb (Cat. #AJ1532a).
  • IHC - Neurofilament M Antibody AJ1532a
    Immunohistochemical analysis of paraffin-embedded human normal brain tissue using anti-Neurofilament M RabMAb (Cat. #AJ1532a).
  • IHC - Neurofilament M Antibody AJ1532a
    Immunohistochemical analysis of paraffin-embedded human glioma using anti-Neurofilament M RabMAb (Cat. #AJ1532a).
  • IF - Neurofilament M Antibody AJ1532a
    Immunofluorescent staining of SH-SY-5Y cells using anti-Neurofilament M RabMAb (Cat. #AJ1532a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession P07197
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2460
Calculated MW 102472 Da
Gene ID 4741
Other Names Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, NEFM, NEF3, NFM
Target/Specificity A synthetic peptide corresponding to residues in human Neurofilament M was used as an immunogen.
Dilution WB~~1:1000~10000
IHC~~1:250~500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament M Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEFM
Synonyms NEF3, NFM
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Research Areas
Citations (0)

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Background

Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-M and NF-L and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2).

References

1. Nixon, R.A., et al. Cell Motil. Cytoskeleton 22, 81-91, 1992 2. Julien, JP., et al. Brain Res. 387, 243-250, 1986

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Discontinued
Cat# AJ1532a
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