Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Amyotrophic Lateral Sclerosis (ALS)   >   Neurofilament M Antibody Phospho (pS614/619)   

Neurofilament M Antibody Phospho (pS614/619)

Rabbit Monoclonal Antibody

  • WB - Neurofilament M Antibody Phospho (pS614/619) AJ1532b
    A. Western blot analysis on SH-SY5Y cell lysates using anti- NF-M (pS614/619) RabMAb (Cat. #AJ1532b), 1:500 dilution.
  • IHC - Neurofilament M Antibody Phospho (pS614/619) AJ1532b
    B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NF-M RabMAb (Cat. #AJ1532b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07197
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR580(2)Y
Calculated MW 102472 Da
Gene ID 4741
Other Names Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, NEFM, NEF3, NFM
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament M Antibody Phospho (pS614/619) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms NEF3, NFM
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). Several phsopholyation sites have been discovered on NF-M, including serine 614 and 619. However, their specific implication on NF-M function has not been identified.


1. Nixon, R.A., and Shea, T. B. (1992) Cell Motil. Cytoskeleton 22, 81-91
2. Julien, JP., Meyer, D., Flavell, D., Hurst, J., and Grosveld, F. (1986) Brain Res. 387, 243-250
3. Kim SK et al. J Neurosci Methods Dec 2006.
4. Lin H et al. Hum Mol Genet 14(23):3643-59, 2005.

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# AJ1532b
(40 western blots)
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions