|Application ||WB, IHC|
|Calculated MW||102472 Da|
|Other Names||Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, NEFM, NEF3, NFM|
|Target/Specificity||A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Neurofilament M Antibody Phospho (pS614/619) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.|
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Provided below are standard protocols that you may find useful for product applications.
Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). Several phsopholyation sites have been discovered on NF-M, including serine 614 and 619. However, their specific implication on NF-M function has not been identified.
1. Nixon, R.A., and Shea, T. B. (1992) Cell Motil. Cytoskeleton 22, 81-91
2. Julien, JP., Meyer, D., Flavell, D., Hurst, J., and Grosveld, F. (1986) Brain Res. 387, 243-250
3. Kim SK et al. J Neurosci Methods Dec 2006.
4. Lin H et al. Hum Mol Genet 14(23):3643-59, 2005.
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