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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Amyotrophic Lateral Sclerosis (ALS)   >   Neurofilament M Antibody Phospho (pS614/619)   

Neurofilament M Antibody Phospho (pS614/619)

Rabbit Monoclonal Antibody

  • WB - Neurofilament M Antibody Phospho (pS614/619) AJ1532b
    A. Western blot analysis on SH-SY5Y cell lysates using anti- NF-M (pS614/619) RabMAb (Cat. #AJ1532b), 1:500 dilution.
  • IHC - Neurofilament M Antibody Phospho (pS614/619) AJ1532b
    B. Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-NF-M RabMAb (Cat. #AJ1532b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P07197
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EPR580(2)Y
Calculated MW 102472 Da
Gene ID 4741
Other Names Neurofilament medium polypeptide, NF-M, 160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, NEFM, NEF3, NFM
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament M Antibody Phospho (pS614/619) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms NEF3, NFM
Function Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Research Areas
Citations (0)

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Neuofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). Several phsopholyation sites have been discovered on NF-M, including serine 614 and 619. However, their specific implication on NF-M function has not been identified.


1. Nixon, R.A., and Shea, T. B. (1992) Cell Motil. Cytoskeleton 22, 81-91
2. Julien, JP., Meyer, D., Flavell, D., Hurst, J., and Grosveld, F. (1986) Brain Res. 387, 243-250
3. Kim SK et al. J Neurosci Methods Dec 2006.
4. Lin H et al. Hum Mol Genet 14(23):3643-59, 2005.

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Cat# AJ1532b
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