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Neurofilament-66 Antibody

Rabbit Monoclonal Antibody

  • WB - Neurofilament-66 Antibody AJ1533a
    A. Western blot analysis on SHSY5Y cell lysate using anti-NF-66 RabMAb (Cat. #AJ1533a).
  • IF - Neurofilament-66 Antibody AJ1533a
    B. Immunofluorescent staining of PC 12 cells using anti-NF-66 RabMAb (Cat. #AJ1533a)
  • IHC - Neurofilament-66 Antibody AJ1533a
    C. Positive immunohistochemical staining of paraffin-embedded human brain tissue (A.) using anti-NF-66 RabMAb.
  • IHC - Neurofilament-66 Antibody AJ1533a
    D. Negative immunohistochemical staining of paraffin-embedded (D) human colon (E), Kidney (F) and uterus (D) tissues using NF-66 RabMAb (Cat#. 1674-1). Tissues were stained in parallel on the same Normal Tissue Array
  •  - Neurofilament-66 Antibody AJ1533a
    E. Kidney
  •  - Neurofilament-66 Antibody AJ1533a
    F. Uterus
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16352
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP676Y
Calculated MW 55391 Da
Gene ID 9118
Other Names Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
Target/Specificity A synthetic peptide corresponding to residues in the tail domain of human NF-66 was used as immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNeurofilament-66 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name INA
Synonyms NEF5
Function Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Tissue Location Found predominantly in adult CNS.
Research Areas
Citations (0)

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Neurofilaments (NF) are cytoplasmic intermediate filaments (IFs) found in neurons. NF-66, also known as α-internexin, is a member of the IF family. Classified as a type IV IF, NF-66 is expressed by most neurons and has been shown to co-assemble with other NF triple proteins (light, medium and heavy NF) (1-2). In the adult brain, NF-66 is expressed at relatively low levels compared to the other NF proteins (3). NF-66 has also been shown to assemble into 10mm diameter IF in vitro.


1. Kaplan MP, Chin SSM, Fliegner KH, Liem RKH: J Neurosci 1990, 10:2735-2748
2. Ching GY, Liem RKH: J Biol Chem 1991, 29:19459-68
3. Ching GY, Liem RKH: J Cell Sci 1998, 111:321-333

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Cat# AJ1533a
(40 western blots)
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