|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||112479 Da|
|Other Names||Neurofilament heavy polypeptide, NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein, NEFH, KIAA0845, NFH|
|Target/Specificity||A synthetic peptide corresponding to residues in human NF-H was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NF-H Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.|
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Provided below are standard protocols that you may find useful for product applications.
Neurofilaments (NF) are members of the intermediate filament protein family also known as 10nm filaments. These neuron-specific filaments are the major constituents of the axonal cytoskeleton (1). NFs are composed of three major proteins; NF-H, NF-L and NF-M and the ratio of each protein changes during neuronal development. NF-H is the heavy molecular weight protein (200 kDa), NF-M the medium (160 kDa) and NF-L the light protein (68kDa) (2). Phosphorylation of the NF-H tail domain modulates neurofilament transport, dynamics and organization within the axon. Accumulation of the protein or mutations in NF-H is observed during the progression of numerous neurological diseases such as amyotrophic lateral sclerosis (3).
1. Nixon, R. A., and Shea, T. B. (1992) Cell Motil. Cytoskeleton 22, 81-91
2. Julien, J. P., Meyer, D., Flavell, D., Hurst, J., and Grosveld, F. (1986) Brain Res. 387, 243-250
3. Al-Chalabi et al. Hum. Molec. Genet. 8: 157-164, 1999.
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