|Reactivity||Human, Mouse, Rat|
|Calculated MW||26959 Da|
|Other Names||Beta-nerve growth factor, Beta-NGF, NGF, NGFB|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human NGF-beta precursor (pro-NGF) form was used as an immunogen. This antibody recognizes pro-NGF.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NGF beta Antibody (precursor) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).|
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Provided below are standard protocols that you may find useful for product applications.
Nerve growth factor (NGF) stimulates division and differentiation of sympathetic and embryonic sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurons (1). NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. While NGF is found in almost all vertebrates, most research has focused on murine NGF, as the mouse male submaxillary gland contains higher levels of this polypeptide than other tissues (2). A precursor form of NGF (pro-NGF) selectively binds to p75NTR receptor and leads to apoptosis with expression of TrkA and p75NTR . However, mature NGF binding to TrkA and p75NTR receptors shows opposite effect and leads to activation of cell survival (3).
1. Levi-Montalcini R. Science 237(4819):1154-62, 1997.
2. Ullrich A, et al. Nature 303(5920):821-5, 1983.
3. Lee, R et al. Science 294:1945, 2001
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