|Reactivity||Human, Mouse, Rat|
|Calculated MW||78411 Da|
|Other Names||Nicastrin, NCSTN, KIAA0253|
|Target/Specificity||A synthetic peptide corresponding to residues on the C-terminus of human Nicastrin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Nicastrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:10993067, PubMed:12679784, PubMed:25043039, PubMed:26280335). The gamma- secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.|
|Cellular Location||Membrane; Single-pass type I membrane protein Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
|Tissue Location||Detected in brain (at protein level) (PubMed:10993067). Widely expressed (PubMed:11396676)|
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Provided below are standard protocols that you may find useful for product applications.
Nicastrin, a transmembrane glycoprotein, is an essential component of the gamma-secretase complex that is required for the intramembrane proteolysis of an increasing number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch (1). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex (2). Nicastrin regulates gamma-secretase cleavage of the amyloid precursor protein by forming complexes with presenilins, in which most mutations causing familial early-onset Alzheimer's disease (EOAD) have been found (3).
1. Yu G, et al. Nature 407(6800):48-54, 2000
2. The UniProt Consortium. The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. D142-D148 (2010).
3. Dermaut B, et al. Am J Hum Genet 70(6):1568-74, 2002
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