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Nicastrin Antibody

Rabbit Monoclonal Antibody

  • WB - Nicastrin Antibody AJ1544a
    A. Western blot analysis on (A) HeLa (B) HL-60 and (C) Jurkat cell lysate using anti-Nicastrin RabMAb (Cat. #AJ1544a), dilution 1:1000.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q92542
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR2575Y
Calculated MW 78411 Da
Gene ID 23385
Other Names Nicastrin, NCSTN, KIAA0253
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human Nicastrin was used as an immunogen.
Dilution WB~~1:1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNicastrin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIAA0253
Function Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
Cellular Location Membrane; Single-pass type I membrane protein. Melanosome Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV
Tissue Location Widely expressed.
Research Areas
Citations (0)

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Nicastrin, a transmembrane glycoprotein, is an essential component of the gamma-secretase complex that is required for the intramembrane proteolysis of an increasing number of membrane proteins, including the amyloid-beta precursor protein (APP) and Notch (1). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex (2). Nicastrin regulates gamma-secretase cleavage of the amyloid precursor protein by forming complexes with presenilins, in which most mutations causing familial early-onset Alzheimer's disease (EOAD) have been found (3).


1. Yu G, et al. Nature 407(6800):48-54, 2000
2. The UniProt Consortium. The Universal Protein Resource (UniProt) in 2010. Nucleic Acids Res. D142-D148 (2010).
3. Dermaut B, et al. Am J Hum Genet 70(6):1568-74, 2002

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Cat# AJ1544a
(40 western blots)
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