|Application ||WB, IHC|
|Calculated MW||17149 Da|
|Other Names||Nucleoside diphosphate kinase A, NDK A, NDP kinase A, Granzyme A-activated DNase, GAAD, Metastasis inhibition factor nm23, NM23-H1, Tumor metastatic process-associated protein, NME1, NDPKA, NM23|
|Target/Specificity||A synthetic peptide corresponding to residues in human Nm23-H1 (NDPK-A) was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Nm23-H1 Antibody (NDPK-A) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. During GZMA-mediated cell death, works in concert with TREX1. NME1 nicks one strand of DNA and TREX1 removes bases from the free 3' end to enhance DNA damage and prevent DNA end reannealing and rapid repair.|
|Cellular Location||Cytoplasm. Nucleus. Note=Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA|
|Tissue Location||Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation|
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Provided below are standard protocols that you may find useful for product applications.
NM23-H1 (NDPK-A, NME1) is a nucleoside diphosphate kinase that is responsible for the synthesis of nucleoside triphosphates and is involved in numerous regulatory processes associated with proliferation, development, and differentiation (1). Evidence points to NM23-H1 (NDPK-A) functioning as a metastasis promoter in other human tumors including neuroblastoma (2).
1. Munier A, et al. Exp Cell Res. 289(2):295-306, 2003
2. Almgren M., et al. Molecular Cancer Research 2:387-394, 2004
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