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nNOS Antibody

Rabbit Monoclonal Antibody

  • WB - nNOS Antibody AJ1548a
    A. Western blot analysis on mouse brain tissue lysate using anti-nNos RabMAb (Cat. #AJ1548a), dilution 1:1000
  • IHC - nNOS Antibody AJ1548a
    B. Immunohistochemical analysis of paraffin-embedded human heart muscle using anti-nNOS RabMAb (Cat. #AJ1548a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29475
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1855Y
Calculated MW 160970 Da
Gene ID 4842
Other Names Nitric oxide synthase, brain, Constitutive NOS, NC-NOS, NOS type I, Neuronal NOS, N-NOS, nNOS, Peptidyl-cysteine S-nitrosylase NOS1, bNOS, NOS1
Target/Specificity A synthetic peptide corresponding to residues around serine 1417 of human nNOS protein was used as an immunogen.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsnNOS Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NOS1
Function Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Cellular Location Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).
Tissue Location Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland
Research Areas
Citations (0)

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NOS (nitric oxide synthase) is a mediator of NO (nitric oxide) production, an inorganic gaseous messenger between cells. In cells, NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Three isoforms of NOS has been identified (eNOS, iNOS, and nNOS) with all of them having binding sites for NADPH, FAD, FMN, and tetrahydrobiopterin (1). Specifically, nNOS (Neuronal nitric oxide synthase bNOS, cNOS, Type I) is bound to PSD95 located in the neuronal membrane; at increased level of Ca2+, nNOS is translocated to the cytoplasm. In the cytoplasm, nNOS is dephosphorylated by calcineurin, which initiates NO production (2). Phosphorylation by PKA and PKC on nNOS leads to downregulation of NO production.


1. Dawson TM et al Prog. Brain Res., 118:3-11, 1998.
2. Watanabe Y et al. Biochem. J. 372:465-471, 2003.

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Cat# AJ1548a
(40 western blots)
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