|Calculated MW||39561 Da|
|Other Names||Nodal homolog, NODAL|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human Nodal was used as an immunogen. This antibody detects Pro-Nodal and Nodal.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Nodal Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential for mesoderm formation and axial patterning during embryonic development.|
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Vertebrates have characteristic and conserved left-right (L-R) visceral asymmetries, for example the left-sided heart. In humans, alterations of L-R development can have serious clinical implications, including cardiac defects. Results support a key role for nodal and nodal-related genes in interpreting and relaying L-R patterning information in vertebrates (1). Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, it has been shown that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination (2). During gastrulation, the three germ layers of the embryo are formed and organized along the anterior-posterior body axis. In the mouse, gastrulation involves the delamination of ectodermal cells through the primitive streak and their differentiation into mesoderm. The nodal signaling molecule is essential for mesoderm formation and subsequent organization of axial structures in early mouse development (3).
1. Raya A, et al. Genes Dev 17(10):1213-8, 2003
2. Lowe LA, et al. Nature 381(6578):158-61, 1996
3. Zhou X, et al. Nature 361(6412):543-7, 1993
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