|Application ||WB, IHC|
|Calculated MW||67827 Da|
|Other Names||Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1, Nuclear factor, erythroid derived 2, like 2, NFE2L2, NRF2|
|Target/Specificity||A synthetic peptide corresponding to residues near the serine 40 of human Nrf2 protein.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Nrf2 Antibody Phospho (pS40) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.|
|Cellular Location||Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents|
|Tissue Location||Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle|
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Provided below are standard protocols that you may find useful for product applications.
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress (1). Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin (2). Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process (3).
1. MP Chan, et al. Proc Nat Acad Sci. 91:9926-9930, 1994
2. SA Chanas et al. Biochem. J. 365, 405-416, 2002.
3. S Braun et al. Mol. Cell. Biol. 22, 5492-5505, 2002
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