|Application ||WB, FC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||64463 Da|
|Other Names||Nuclear receptor subfamily 4 group A member 1, Early response protein NAK1, Nuclear hormone receptor NUR/77, Nur77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3, NR4A1, GFRP1, HMR, NAK1|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human Nur77 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Nur77 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||GFRP1, HMR, NAK1|
|Function||Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'- AAAAGGTCA-3' (By similarity). May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation.|
|Cellular Location||Cytoplasm. Nucleus.|
|Tissue Location||Fetal muscle and adult liver, brain and thyroid|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Nur77 (TR3, NGFI-B) is an orphan nuclear receptor belonging to the steroid/thyroid hormone receptor superfamily with multiple cell functions relating to apoptosis, cell differentiation, and proliferation. Nur77 expression is rapidly induced by several growth factors, mitogens, and stress factors (1). With its central DNA binding domain, Nur77 binds to specific DNA elements and regulates the transcription of the target gene (2). While Nur77 activities are shown to be specific to its sub-cellular location, the phospholyation on Nur77 by various kinases (e.g. RSK, MSK) might affect its transactivation activity and its localization (3, 4). Nur77 localized in the nucleus functions as an oncogenic survival factor and promotes cell growth. However, in mitochondria, it functions as an apoptosis factor after binding to Bcl-2 and triggering cytochrome c release. Nur77 has been linked to apoptosis of immature T cell and T cell hybridoma. In tumors, it is involved in both apoptosis and proliferation of cancer cells (4).
1. Kolluri SK, Mol Cell Biol. 23(23):8651-67, 2003. 2. Wilson TE et al., Science 252:1296-1300, 1993. 3. Wingate WD et al., Biochem J. 393(Pt 3): 715?724, 2006 4 Zhang XK. Expert Opin Ther Targets. 11(1):69-79, 2007.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.