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>   home   >   Products   >   Primary Antibodies   >   Immunology   >   OCA-B Antibody   

OCA-B Antibody

Rabbit Monoclonal Antibody

  • WB - OCA-B Antibody AJ1557a
    A. Western blot analysis on Ramos cell lysate using anti-OCA-B RabMAb (Cat. #AJ1557a), dilution 1:30,000.
  • IHC - OCA-B Antibody AJ1557a
    B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-OCA-B RabMAb (Cat. #AJ1557a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16633
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP3300
Calculated MW 27436 Da
Gene ID 5450
Other Names POU domain class 2-associating factor 1, B-cell-specific coactivator OBF-1, BOB-1, OCA-B, OCT-binding factor 1, POU2AF1, OBF1
Target/Specificity A synthetic peptide corresponding to residues in human OCA-B was used as an immunogen.
Dilution WB~~1:5000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOCA-B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU2AF1
Synonyms OBF1
Function Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.
Cellular Location Nucleus.
Tissue Location B-cell specific.
Research Areas
Citations (0)

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OCA-B is a lymphoid-specific transcriptional coactivator which interacts with the transcription factors Oct-1 and Oct-2 (1). It has been shown to be critical for development of a normal immune response, where it mediates octamer-dependent transcriptional activity in B lymphocytes. It is also critically involved in the formation of germinal centers in secondary lymphoid organs. OCA-B levels have been observed to be massively upregulated in germinal center B cells, as compared with resting B cells. SIAH1 and SIAH2, known regulators of protein stability, have been identified as two related OCA-B interacting proteins. SIAH1 has been suggested as part of a novel regulatory loop controlling the level of OCA-B protein in B cells. B-cell receptor cross-linking results in a degradation of OCA-B, which further results in a reduction of transcriptional activation of OCA-B-dependent reporters (2, 3).


1. Bartholdy B, et al. Proc Natl Acad Sci U S A. 103(31):11665-70, 2006
2. Tiedt R, et al. EMBO J. 20(15):4143-52, 2001
3. Boehm J, et al. EMBO J. 20(15):4153-62, 2001

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Cat# AJ1557a
(40 western blots)
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