|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||16320 Da|
|Other Names||Actin-related protein 2/3 complex subunit 5, Arp2/3 complex 16 kDa subunit, p16-ARC, ARPC5, ARC16|
|Target/Specificity||A synthetic peptide corresponding to residues on the N-terminus of human p16-ARC was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||p16-Arc Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell projection|
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Provided below are standard protocols that you may find useful for product applications.
The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells. The human complex consists of seven subunits which include the actin related proteins Arp2 and Arp3, and five others referred to as p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc (p complex) (1). These proteins localize in the cytoplasm of fibroblasts that lack lamellipodia, but are enriched in lamellipodia on stimulation with serum or platelet-derived growth factor. A dynamic role for this complex in the organization of the actin cytoskeleton has been suggested (2). p16-Arc has been found to be a globular alpha-helical subunit (3).
1. Welch MD, et al. J Cell Biol 138(2):375-84, 1997
2. Machesky LM, et al. Biochem J. 328(Pt1):105-12, 1997
3. Robinson RC, et al. Science 294(5547):1679-84, 2001
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