|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||172460 Da|
|Other Names||Rho GTPase-activating protein 5, Rho-type GTPase-activating protein 5, p190-B, ARHGAP5, RHOGAP5|
|Target/Specificity||A synthetic peptide corresponding to residues in human p190-B RhoGAP was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||p190-B RhoGAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||GTPase-activating protein for Rho family members (PubMed:8537347).|
|Cellular Location||Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Also membrane-associated in a fibrillar pattern that colocalizes with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin|
|Tissue Location||Detected in skin fibroblasts (at protein level) (PubMed:8537347).|
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Provided below are standard protocols that you may find useful for product applications.
The p190 Rho GTPase activating protein RhoGAP) family includes two closely related proteins, p190-A and p190-B. p190-B contains an N-terminal GTPase domain and a C-terminal Rho-GAP domain (1). p190-B can be directly phosphorylated on a single identified tyrosine residue by the activated insulin and IGF-1 receptors. This phosphorylation causes p190-B to translocate to the lipid rafts of the plasma membrane, where it can facilitate the inactivation of the Rho GTPase. P190-B also has been shown to be tyrosine phosphorylated by c-Src and v-Src. Additionall; the GAP domain of p190-B as shown to attenuate signal-transducing activity of Rac, Rho and CDC42 (2-3).
1. Burbelo et al. J. Biol. Chem. 270: 30919-30926, 1995.
2. Peter et al. Volume 270, Number 52, Issue of December 29, 1995 pp. 30919-30926
3. DZ Wang, MS Nur-E-Kamal, A Tikoo, W Montague and H Maruta. Cancer Research, Vol 57, Issue 12 2478-2484.
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