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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   p27/Kip1 Antibody   

p27/Kip1 Antibody

Rabbit Monoclonal Antibody

  • WB - p27/Kip1 Antibody AJ1569a
    Western blot analysis on (A) NIH/3T3, (B) C6 and (C) Neuro-2a cell lysates using anti-p27/Kip1 RabMAb (Cat. #AJ1569a).
  • IHC - p27/Kip1 Antibody AJ1569a
    Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p27 RabMAb (Cat. #AJ1569a).
  • IHC - p27/Kip1 Antibody AJ1569a
    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue using anti-p27 RabMAb (Cat. #AJ1569a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P46414
Reactivity Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPFHCR16
Calculated MW 22193 Da
Gene ID 12576
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, Cdkn1b
Target/Specificity A recombinant protein in mouse p27/Kip1was used as an immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp27/Kip1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Cdkn1b
Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of cyclin D-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-197, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser- 10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 (By similarity). Colocalizes at the endosome with SNX6; this leads to lysosomal degradation
Research Areas
Citations (0)

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p27/Kip1 is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27/Kip1 binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27/Kip1 is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27/Kip1 (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).


1. Polyak K., et al. Cell 78:59-66, 1994. 2. Carrano,A.C., et al. Nat. Cell Biol., 1, 193-199, 1999 3. Rodier,G., et al. EMBO J., 20, 6672-6682, 2001

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Cat# AJ1569a
(40 western blots)
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