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p27/Kip1 Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AJ1569c | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
p27/Kip1 Antibody (C-term) - Product info | |
| Application | WB, IHC, IF
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| Primary Accession | P46527 |
| Reactivity | Human, Rat |
| Clone Names | Y236 |
| Calculated MW | 22073 Da |
| Gene ID 1027 | |
| Other Names CDKN1B, KIP1, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27;p27Kip1 | |
| Target/Specificity A synthetic peptide corresponding to residues in the C-term of human p27(Kip1) was used as immunogen | |
| Dilution WB~~1:500 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions p27/Kip1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
p27/Kip1 Antibody (C-term) - Protein Information | |
| Name CDKN1B | |
| Synonyms KIP1 | |
| Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry | |
| Cellular Location Nucleus. Cytoplasm. Endosome (By similarity). Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity) | |
| Tissue Location Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney | |
p27/Kip1 Antibody (C-term) - Related products
AP10418a: 27Kip1-T157 Antibody
AP13302b: CDKN1B Antibody (C-term)
AP3191a: Phospho-p27Kip1-S10 Antibody
AP3192a: Phospho-p27Kip1-S178 Antibody
AP3193a: Phospho-p27Kip1-T157 Antibody
AP3194a: Phospho-p27Kip1-T187 Antibody
AP3529a: Phospho-p27Kip1-T198 Antibody
AP6269b: 27Kip1 Antibody (C-term)
RI10983: CDKN1B predesign siRNA
LY12364a: CDKN1B Over-expression Lysate
BP10418a: 27Kip1-T157 Antibody Blocking peptide
BP3191a: Phospho-p27Kip1-S10 Antibody Blocking Peptide
BP3192a: Phospho-p27Kip1-S178 Antibody Blocking Peptide
BP3193a: Phospho-p27Kip1-T157 Antibody Blocking Peptide
BP3194a: Phospho-p27Kip1-T187 Antibody Blocking Peptide
BP3529a: Phospho-p27Kip1-T198 Antibody Blocking Peptide
BP6269b: 27Kip1 Antibody (C-term) Blocking Peptide
AT1481a: CDKN1B Antibody (monoclonal) (M01)
AJ1569c: p27/Kip1 Antibody (C-term)
AJ1569d: p27/Kip1 Antibody Phospho (pS10)
p27/Kip1 Antibody (C-term) - Application data
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A. Western blot analysis on MCF-7 cell lysate using anti-p27 (Kip1) RabMAb (Cat. #AJ1569c), 1:1,000 dilution.
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B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-p27 (Kip1) (C-term) RabMAb (Cat. #AJ1569c).
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C. Immunofluorescent staining of PC-12 cells using anti-P27 (Kip1) (C-term) RabMAb (Cat. #AJ1569c).
p27/Kip1 Antibody (C-term) - Research Areas
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BACKGROUND
p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).
REFERENCES
1. Polyak K., Lee M.-H., Erdjument-Bromage H., Koff A., Roberts J.M., Tempst P., Massague J.; Cell 78:59-66 (1994).
2. Carrano,A.C., Eytan,E., Hershko,A. and Pagano,M. (1999) SKP2 is required for ubiquitin-mediated degradation of the CDK inhibitor p27. Nat. Cell Biol., 1, 193-199
3. Rodier,G., Montagnoli,A., Di Marcotullio,L., Coulombe,P., Draetta,G.F., Pagano,M. and Meloche,S. (2001). EMBO J., 20, 6672-6682
