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p27/Kip1 Antibody Phospho (pS10)

Rabbit Monoclonal Antibody

  • WB - p27/Kip1 Antibody Phospho (pS10) AJ1569d
    A. Western blot analysis on MCF-7 cell lysates using anti-Phospho-p27 Kip1 (pS10) RabMAb (Cat. #AJ1569d), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP.
  • IHC - p27/Kip1 Antibody Phospho (pS10) AJ1569d
    C. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-Phospho-p27Kip1 RabMAb. (Cat. #AJ1569d).
  • IHC - p27/Kip1 Antibody Phospho (pS10) AJ1569d
    B. Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using anti-Phospho-p27Kip1 RabMAb (Cat. #AJ1569d)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P46527
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP233(2)Y
Calculated MW 22073 Da
Gene ID 1027
Other Names Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1, CDKN1B, KIP1
Target/Specificity A phospho specific peptide corresponding to residues surrounding Serine 10 of human p27 was used as an immunogen. This antibody detects p27 phosphorylated at Serine 10.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp27/Kip1 Antibody Phospho (pS10) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms KIP1
Function Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1- CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
Cellular Location Nucleus. Cytoplasm. Endosome. Note=Nuclear and cytoplasmic in quiescent cells. AKT- or RSK- mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen- activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).
Tissue Location Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney
Research Areas
Citations (0)

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p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 (1). p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1) (2); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm (3).


1. Polyak K., et al; Cell 78:59-66,1994.
2. Carrano,A.C., et al. Nat. Cell Biol., 1, 193-199
3. Rodier,G.,et al. EMBO J., 20, 6672-6682, 2001

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Cat# AJ1569d
(40 western blots)
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