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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   p58 Antibody   

p58 Antibody

Rabbit Monoclonal Antibody

     
  • WB - p58 Antibody AJ1575a
    A. Western blot analysis on HeLa cell lysate using anti-p58 RabMAb (Cat. #AJ1575a), dilution 1:5000
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession Q13217
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP597Y
Calculated MW 57580 Da
Gene ID 5611
Other Names DnaJ homolog subfamily C member 3, Endoplasmic reticulum DNA J domain-containing protein 6, ER-resident protein ERdj6, ERdj6, Interferon-induced, double-stranded RNA-activated protein kinase inhibitor, Protein kinase inhibitor of 58 kDa, Protein kinase inhibitor p58, DNAJC3, P58IPK, PRKRI
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human p58 was used as an immunogen.
Dilution WB~~1:5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautionsp58 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DNAJC3
Synonyms P58IPK, PRKRI
Function Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions (By similarity). Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
Cellular Location Endoplasmic reticulum.
Tissue Location Widely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
Research Areas
Citations (0)

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Background

The 58-kDa inhibitor of the interferon-induced double-stranded RNA-activated protein kinase (PKR) is a cellular protein that is activated during influenza virus infection to down-regulate the activity of PKR. The 58-kDa inhibitor of the interferon-induced double-stranded RNA-activated protein kinase (PKR) is a cellular protein that is activated during influenza virus infection to down-regulate the activity of PKR (1). The P58 protein inhibits both the autophosphorylation of PKR and the phosphorylation of the PKR natural substrate, the alpha subunit of eukaryotic initiation factor eIF-2. Sequence analysis revealed that P58 is a member of the tetratricopeptide family of proteins (2). Also, like other J-domain proteins, P58 stimulated the ATPase activity of Hsc70. Taken together, data suggests that P58 is a co-chaperone, possibly directing hsp/Hsc70 to refold, and thus inhibit kinase function (3).

References

1. Korth MJ, et al. Gene 170(2):181-8, 1996.
2. Polyak SJ, et al. J Biol Chem. 271(3):1702-7, 1996.
3. Melville MW, et al. J Biol Chem. 274(6): 3797-803, 1999.

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Discontinued
Cat# AJ1575a
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