|Application ||WB, IHC|
|Calculated MW||32749 Da|
|Other Names||Polyadenylate-binding protein 2, PABP-2, Poly(A)-binding protein 2, Nuclear poly(A)-binding protein 1, Poly(A)-binding protein II, PABII, Polyadenylate-binding nuclear protein 1, PABPN1, PAB2, PABP2|
|Target/Specificity||A synthetic peptide corresponding to residues near the N-terminus of human PABP2 was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PABP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation (By similarity).|
|Cellular Location||Nucleus. Cytoplasm Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic Nucleus; nuclear speckle. Colocalizes with SKIP and poly(A) RNA in nuclear speckles (By similarity).|
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Poly (A)-binding protein II (PABP2) is an abundant nuclear protein that binds with high affinity to nascent poly (A) tails, stimulating their extension and controlling their length. PABP2 is reported to interact with the nuclear export machinery and may therefore contribute to mRNA transport (1). PABP2 stimulates extension of the growing poly (A) tail during the polyadenylation process, and limits the length of the newly synthesized poly (A) tail (2). PABP2 is reported to be detected in filamentous nuclear inclusions, which are the pathological hallmark of oculopharyngeal muscular dystrophy. This suggests that the polyalanine expansions in PABP2 induce a misfolding and aggregation of the protein into insoluble inclusions, similarly to events in neurodegenerative diseases caused by CAG/polyglutamine expansions (3).
1. Calado A, et al. RNA 6(2):245-56, 2000
2. Kim YJ, et al. Hum Mol Genet. 10(11):1129-39, 2001
3. Calado A, et al. Hum Mol Genet. 9(15):2321-8, 2000
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