|Calculated MW||64505 Da|
|Other Names||Paxillin, PXN|
|Target/Specificity||A synthetic phospho-peptide corresponding to residues surrounding Tyr181 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 181.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Paxillin Antibody Phospho (pY181) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery.|
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Provided below are standard protocols that you may find useful for product applications.
Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).
1. Salgia, R., et al. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J Biol Chem. 270: 5039-47 (1995).
2. Burridge, K., et al. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119: 893-903 (1992).
3. . Bellis, S.L., et al. Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase. J. Biol. Chem. 270: 17437-17441 (1995).
4. Iwasaki, T., et al. Involvement of phosphorylation of Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration. Int J Cancer. 97: 330-5 (2002).
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