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Paxillin Antibody Phospho (pY31)

Rabbit Monoclonal Antibody

  • WB - Paxillin Antibody Phospho (pY31) AJ1592c
    A. Western blot analysis on NIH 3T3 cell lysate using anti-Phospho-Paxillin (pY31)RabMAb (Cat. #AJ1592c); dilution 1:10,000. Cells were (1) untreated, and (2) treated with PDGF.
  • IHC - Paxillin Antibody Phospho (pY31) AJ1592c
    B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-Phospho-Paxillin (pY31) RabMAb (Cat. #AJ1592c).
  • IF - Paxillin Antibody Phospho (pY31) AJ1592c
    C. Immunofluorescent staining of HeLa cells using anti-Phospho-Paxillin (pY31) RabMAb (Cat. #AJ1592c).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P49023
Reactivity Human, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E228
Calculated MW 64505 Da
Gene ID 5829
Other Names Paxillin, PXN
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr31 of human Paxillin was used as immunogen. The antibody only detects Paxillin phosphorylated on Tyrosine 31.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPaxillin Antibody Phospho (pY31) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PXN
Function Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).
Cellular Location Cytoplasm, cytoskeleton. Cell junction, focal adhesion. Cytoplasm, cell cortex. Note=Colocalizes with integrins at the cell periphery.
Research Areas
Citations (0)

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Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion) (1). Paxillin plays a significant role in integrin signaling and the tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization (2). Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118 (3). It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration (4).


1. Salgia, R., et al. Molecular cloning of human paxillin, a focal adhesion protein phosphorylated by P210BCR/ABL. J Biol Chem. 270: 5039
2. Burridge, K., et al. Tyrosine phosphorylation of paxillin and pp125FAK accompanies cell adhesion to extracellular matrix: a role in cytoskeletal assembly. J. Cell Biol. 119: 893
3. Bellis, S.L., et al. Characterization of tyrosine phosphorylation of paxillin in vitro by focal adhesion kinase. J. Biol. Chem. 270: 17437
4. Iwasaki, T., et al. Involvement of phosphorylation of Tyr-31 and Tyr-118 of paxillin in MM1 cancer cell migration. Int J Cancer. 97: 330

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Cat# AJ1592c
(40 western blots)
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