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PBK (TOPK) Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - PBK (TOPK) Antibody (C-term) AJ1593b
    A. Western blot analysis on HeLa cell lysate using anti-PBK RabMAb (Cat. #AJ1593b), dilution 1:500,000.
  • IHC - PBK (TOPK) Antibody (C-term) AJ1593b
    B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-PBK(TOPK) RabMAb (Cat. #AJ1593b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q96KB5
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2520Y
Calculated MW 36085 Da
Gene ID 55872
Other Names Lymphokine-activated killer T-cell-originated protein kinase, Cancer/testis antigen 84, CT84, MAPKK-like protein kinase, Nori-3, PDZ-binding kinase, Spermatogenesis-related protein kinase, SPK, T-LAK cell-originated protein kinase, PBK, TOPK
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human PBK was used as an immunogen.
Dilution WB~~1:500~2000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPBK (TOPK) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PBK
Synonyms TOPK
Function Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.
Tissue Location Expressed in the testis and placenta. In the testis, restrictedly expressed in outer cell layer of seminiferous tubules.
Research Areas
Citations (0)

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A novel mitotic kinase, PDZ-binding kinase or PBK, which is upregulated in a variety of neoplasms including hematological malignancies, has been the focus of attention with a goal to understand its role in malignant conversion and to examine as a possible new therapeutic target in disparate types of cancer. Studies provide a plausible explanation for the role of PBK augmenting tumor cell growth following transient appearance in different types of progenitor cells in vivo (1). PBK is a newly identified member of a novel MEK3/6-related MAPKK that may be enrolled in the activation of lymphoid cells and support testicular functions (2). In vitro, PBK binds specifically to PDZ2 of hDlg through its C-terminal T/SXV motif. PBK and hDlg are phosphorylated at mitosis in HeLa cells, and the mitotic phosphorylation of PBK is required for its kinase activity. In vitro, cdc2/cyclin B phosphorylates PBK. This evidence shows how PBK could link hDlg or other PDZ-containing proteins to signal transduction pathways regulating the cell cycle or cellular proliferation (3).


1. Nandi AK, et al. Biochem Biophys Res Commun 358(1): 181-1 (2007)
2. Abe Y, et al. J Biol Chem 275(28):21525-31 (2000)
3. Gaudet S, et al. Proc Natl Acad Sci 97(10):5167-72 (2000)

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Cat# AJ1593b
(40 western blots)
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