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PCNA Antibody

Rabbit Monoclonal Antibody

  • WB - PCNA Antibody AJ1594a
    Western blot analysis on (A) HeLa (B) HepG2 (C) 293T and (D) A431 cell lysates using anti-PCNA RabMAb (Cat. #AJ1594a).
  • IHC - PCNA Antibody AJ1594a
    Immunohistochemical analysis of paraffin-embedded tonsil tissue using anti-PCNA RabMAb (Cat. #AJ1594a).
  • IF - PCNA Antibody AJ1594a
    Immunofluorescent staining of HeLa cells using anti-PCNA RabMAb (Cat. #AJ1594a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P12004
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR3821
Calculated MW 28769 Da
Gene ID 5111
Other Names Proliferating cell nuclear antigen, PCNA, Cyclin, PCNA
Target/Specificity A synthetic peptide corresponding to residues in human PCNA was used as an immunogen.
Dilution WB~~1:500~1000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCNA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Cellular Location Nucleus. Note=Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (PubMed:24939902). Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.
Research Areas
Citations ( 0 )


Proliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).


1. Gary R, et al. J Biol Chem 272(39):24522-9, 1997 2. Zhang S, et al. Cell Cycle 7(21):3399-404, 2008

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Cat# AJ1594a
(40 western blots)
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