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> home > Products > Antibodies > Biological Process > Protein Amino Acid Phosphorylation > PDK1 Antibody

PDK1 AntibodyRabbit Monoclonal Antibody

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Catalog #	Size	Availability	Price
AJ1597b	100ul 400 ul	2-3 days	$ 315.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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PDK1 Antibody - Product info
Application	WB, IHC Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	O15530
Reactivity	Human, Mouse
Clone Names	EP569Y
Calculated MW	63152 Da
Gene ID 5170
Other Names Cd79b, Igb, B-cell antigen receptor complex-associated protein beta chain, Ig-beta;B-cell-specific glycoprotein B29;Immunoglobulin-associated B29 protein
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Tyr373 and Tyr376 of human PDK1 was used as immunogen. This antibody will detect both phosphorylated and non-phosphorylated targets.
Dilution WB~~1:1000 IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions PDK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
PDK1 Antibody - Protein Information
Name PDPK1
Synonyms PDK1
Function Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses. Provides negative feedback inhibition to toll-like receptor-mediated NF- kappa-B activation in macrophages. Isoform 3 is catalytically inactive
Cellular Location Cytoplasm. Nucleus. Cell membrane; Peripheral membrane protein. Cell junction, focal adhesion Note=Tyrosine phosphorylation seems to occur only at the cell membrane. Translocates to the cell membrane following insulin stimulation by a mechanism that involves binding to GRB14 and INSR. SRC and HSP90 promote its localization to the cell membrane Its nuclear localization is dependent on its association with PTPN6 and its phosphorylation at Ser-396. Restricted to the nucleus in neuronal cells while in non-neuronal cells it is found in the cytoplasm. The Ser-241 phosphorylated form is distributed along the perinuclear region in neuronal cells while in non- neuronal cells it is found in both the nucleus and the cytoplasm IGF1 transiently increases phosphorylation at Ser-241 of neuronal PDPK1, resulting in its translocation to other cellular compartments. The tyrosine-phosphorylated form colocalizes with PTK2B in focal adhesions after angiotensin II stimulation
Tissue Location Appears to be expressed ubiquitously. The Tyr- 9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast

PDK1 Antibody - Related products

AP3213a: Phospho-PDK1-S241 Antibody

AP3405a: Phospho-PDPK1-S393 Antibody

AP3458a: Phospho-PDPK1-S396 Antibody

AP3466a: Phospho-PDPK1-S241 Antibody

AP3467a: Phospho-PDPK1-S410 Antibody

AP3476a: Phospho-PDPK1-Y9 Antibody

AP7042a: PDK1 (PDPK1) Antibody (N-term H67)

AP7042b: PDK1 (PDPK1) Antibody (C-term)

AP7042c: PDPK1 Antibody (S396)

AP7042d: PDK1 (PDPK1) Antibody (N-term P71)

AP7042e: PDK1 (PDPK1) Antibody (S241)

AP7042f: PDK1 (PDPK1) Antibody (S410)

AP7042g: PDK1 (PDPK1) Antibody (Y9)

AP7042h: PDPK1 Antibody (S393)

RI13899: PDPK1 predesign siRNA

LY11499a: PDPK1 Over-expression Lysate

BP3405a: Phospho-PDPK1-S393 Antibody Blocking Peptide

BP3458a: Phospho-PDPK1-S396 Antibody Blocking Peptide

BP3466a: Phospho-PDPK1-S241 Antibody Blocking Peptide

BP3467a: Phospho-PDPK1-S410 Antibody Blocking Peptide

BP3476a: Phospho-PDPK1-Y9 Antibody Blocking Peptide

BP7042a: PDPK1 Antibody (N-term) Blocking Peptide

BP7042b: PDPK1 Antibody (C-term) Blocking Peptide

BP7042c: PDPK1 Antibody (S396) Blocking Peptide

BP7042d: PDPK1 Antibody (N-term P71) Blocking Peptide

BP7042e: PDPK1 Antibody (S241) Blocking Peptide

BP7042f: PDPK1 Antibody (S410) Blocking Peptide

BP7042g: PDPK1 Antibody (Y9) Blocking Peptide

BP7042h: PDPK1 Antibody (S393) Blocking Peptide

AJ1597b: PDK1 Antibody

PDK1 Antibody - Application data

A. Western blot analysis on 293 cell lysate using anti- PDK1 RabMAb (Cat. #AJ1597b), 1:1000 dilution.
B. Immunohistochemical analysis of human prostate carcinoma using anti-PDK1 RabMAb (Cat. #AJ1597b).

PDK1 Antibody - Research Areas

Antibodies Biological Process Protein Amino Acid Phosphorylation Post-translational Protein Modification Phosphate Metabolic Process Phosphorus Metabolic Process Phosphorylation Protein Modification Process Biopolymer Modification Metabolic Process Primary Metabolic Process Protein Metabolic Process Cellular Protein Metabolic Process Cellular Metabolic Process Cellular Process Positive Regulation Of Biological Process Positive Regulation Of Cellular Process Response To Organic Substance Regulation Of Phosphorylation Response To Chemical Stimulus Regulation Of Phosphate Metabolic Process Regulation Of Phosphorus Metabolic Process Response To Endogenous Stimulus Macromolecule Metabolic Process Intracellular Signaling Cascade Response To Hormone Stimulus Regulation Of Kinase Activity Regulation Of Protein Kinase Activity Regulation Of Transferase Activity Cellular Macromolecule Metabolic Process Regulation Of Molecular Function Positive Regulation Of Molecular Function

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BACKGROUND

3-Phosphoinositide-dependent protein kinase-1 (PDK1) is a serine/threonine protein kinase known to phosphorylate and activate PKB/AKT (Thr308 and Ser473), p70 S6 kinase, RSK, PKA, PKC and SGK. Upon stimulation of receptor tyrosine kinases, PI3K becomes activated and generates the phospholipid second messengers, PtdIns(3,4,5)P 3 and PtdIns(3,4)P 2, which in turn mediate the phosphorylation and activation of PDK1 targets through diverse mechanisms (1-2). It has also been shown that stimulation of cells with pervanadate and IGF-1 results in a significant increase in PDK1 activity and translocation to the plasma membrane along with an increase in tyrosine phosphorylation (3). Only phosphorylation on the activation loop at Ser241 is necessary for PDK1s activity and a single nucleotide mutation abolishes its activity (4).

REFERENCES

1. Alessi et al. Curr. Biol. 7:776-789, 1997
2. Stokoe et al. Science 277:567-570, 1997
3. Jongsun P, et al. J. Biol. Chem. 276(40):37459-71, 2001
4. Casamayor A, Biochem. J. 342, 287-292, 1999.