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PDK2 Antibody

Rabbit Monoclonal Antibody

  • WB - PDK2 Antibody AJ1598a
    A. Western blot analysis on SH-SY-5Y cell lysate using anti-PDK2 RabMAb (Cat. #AJ1598a), dilution 1:1000
  • IHC - PDK2 Antibody AJ1598a
    B. Immunohistochemical analysis of paraffin-embedded human heart muscle using anti-PDK2 RabMAb (Cat. #AJ1598a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q15119
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1947Y
Calculated MW 46154 Da
Gene ID 5164
Other Names [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial, Pyruvate dehydrogenase kinase isoform 2, PDH kinase 2, PDKII, PDK2, PDHK2
Target/Specificity A synthetic peptide corresponding to residues near the N-terminus of human PDK2 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPDK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PDK2
Synonyms PDHK2
Function Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
Cellular Location Mitochondrion matrix.
Tissue Location Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung
Research Areas
Citations (0)

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A member of Pyruvate dehydrogenase kinase (PDK1, 2, 3, and 4), PDK2 is a serine kinase which is involved in the regulation of mammalian glucose metabolism. During aerobic energy metabolism, mitochondrial pyruvate dehydrogeanse complex (PDC) catalyzes decarboxylation of pyruvate to form acetyl-CoA (1). PDK2 inhibits PDC thru phosphorylation of the E1 alpha subunit of PDC and inactivates the complex (2). Highly expressed in heart and skeletal muscles, PDK2 is also responsible for the phosphorylation of Akt at Ser473, which leads to phosphorylation by PDK1 on Thr308 on Akt and full Akt activation (3).


1. Reed LJ, Hackert ML, JBC 265(16):8971-8974, 1990.
2. Kolobova E et al. JBC 358:69-77, 2001.
3. Balendran A et al. Current Biology 9(8):393-404, 1999.

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Cat# AJ1598a
(40 western blots)
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