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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   Phospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody   

Phospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody

Rabbit Monoclonal Antibody

  • WB - Phospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody AJ1602a
    A. Western blot analysis on serum starved (ss)A431 cell lysate using anti-ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (Cat. #AJ1602a); dilution 1:500
  • IHC - Phospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody AJ1602a
    B. Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti- ERK1(pT202/pY204) /ERK2(pT185/pY187) RabMAb (Cat. #AJ1602a)
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P27361
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names E337
Calculated MW 43136 Da
Gene ID 5595
Other Names Mitogen-activated protein kinase 3, MAP kinase 3, MAPK 3, ERT2, Extracellular signal-regulated kinase 1, ERK-1, Insulin-stimulated MAP2 kinase, MAP kinase isoform p44, p44-MAPK, Microtubule-associated protein 2 kinase, p44-ERK1, MAPK3, ERK1, PRKM3
Target/Specificity A synthetic phospho-peptide corresponding to residues surrounding Thr202 and Tyr204 of human ERK1 was used as immunogen. The antibody only detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204, or ERK2 phosphorylated on Threonine 185 and Tyrosine 187.
Dilution WB~~1:5000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Erk1 (pT202/pY204) / Erk2 (pT185/pY187) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAPK3
Synonyms ERK1, PRKM3
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Cellular Location Cytoplasm. Nucleus. Note=Autophosphorylation at Thr-207 promotes nuclear localization
Research Areas
Citations (0)

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Extracellular signal-regulated kinases Erk1 (p44 MAPK) and Erk2 (p42 MAPK) Serine/Threonine protein kinase are part of a subfamily of mitogen-activated protein kinases that participate in diverse cellular functions, including the regulation of cell growth, differentiation, apoptosis and signal transduction (1,2,3). The simultaneous tyrosine/threonine phosphorylation of ERK1 and ERK2 results in their activation. Although ERK1 and ERK2 resemble one another closely in sequence and are activated by similar pathways, they appear to have different tissue distributions (4) and show differential phosphorylation of potential intracellular substrates (5).


1. Charest, D.L, et al. Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1. Mol Cell Biol. 13: 4679
2. Marshall, C.J. Specificity of receptor tyrosine kinase signaling: transient versus sustained extracellular signal-regulated kinase activation. Cell 80: 179
3. Brott, B. K., Alessandrini, A., Largaespada, D. A., Copeland, N. G., Jenkins, N. A., Crews, C. M., and Ercikson, R. L. (1993) Cell Growth Differ. 4, 921-929
4. Boulton T. G. and Cobb M. H. (1991) Identification of multiple extracellular signal-regulated kinases (ERK5) with antipeptideantibodies. Cell Regul. 2, 357
5. Chuang C. F. and Ng S. Y. (1994) Functional divergence of the MAP kinase pathway. ERKI and ERK2 activate specific transcription factors. FEBS Left. 346, 229

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Cat# AJ1602a
(40 western blots)
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