|Calculated MW||6109 Da|
|Other Names||Cardiac phospholamban, PLB, PLN, PLB|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding Serine 16 of human Phospholamban was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospholamban Antibody Phospho (pS16) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.|
|Cellular Location||Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single- pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein|
|Tissue Location||Heart muscle (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Phospholamban is a major substrate for the cAMP-dependent kinase in cardiac muscle. It regulates the activity of the calcium pump of cardiac sarcoplasmic reticulum. It inhibits cardiac muscle sarcoplasmic reticulum Ca2+-ATPase in the unphosphoyrlated state, but inhibition is relieved upon phosphorylation of the protein (1). Beta-adrenergic stimulation results in PLB phosphorylation by both cAMP-dependent protein kinase at Serine 16 and calcium/calmodulin-dependent protein kinase at Serine 17 (2). Phospholamban is expressed in slow twitch skeletal muscle and some smooth muscle as well as in cardiac muscle (1).
1. Fujii, et al. J. Biol. Chem., 266(18):11669-11675, 1991. 2. Wegener et al, J Biol Chem. 1989; 264:11468-11474.
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