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Phospho-Mst4 (pT178)/Mst3 (pT190)/STK25 (pT174) Antibody

Rabbit Monoclonal Antibody

  • WB - Phospho-Mst4 (pT178)/Mst3 (pT190)/STK25 (pT174) Antibody AJ1605a
    A. Western blot analysis on HeLa cell lysates using anti-Phospho- Mst4 (pT178) RabMAb (Cat. #AJ1605a), 1:2000 dilution. Cells were either (A) untreated (B) treated with calyculin A.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P289
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP2123Y
Calculated MW 46529 Da
Gene ID 51765
Other Names Serine/threonine-protein kinase 26, MST3 and SOK1-related kinase, Mammalian STE20-like protein kinase 4, MST-4, STE20-like kinase MST4, Serine/threonine-protein kinase MASK, STK26 (HGNC:18174)
Target/Specificity A phospho specific peptide corresponding to residues surrounding threonine 178 of human MST4, theonine 190 of human MSt 3 and threonine 174 of human STK25.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-Mst4 (pT178)/Mst3 (pT190)/STK25 (pT174) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name STK26 (HGNC:18174)
Function Mediator of cell growth. Modulates apoptosis.
Cellular Location Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Localizes to the Golgi apparatus
Research Areas
Citations (0)

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Novel human Ste20-related kinase Mst4 is highly expressed in placenta, thymus, and peripheral blood leukocytes. Mst4 is biologically active in the activation of MEK/ERK pathway and in mediating cell growth and transformation (1). Wild type and C-terminally truncated forms of Mst4 can both induce apoptosis upon overexpression in mammalian cells that is abrogated by CrmA, suggesting involvement of Mst4 in the apoptotic machinery in mammalian cells (2). Mst4 was found to be expressed in prostate carcinoma tumor samples and cell lines. In addition, expression levels appeared to correlate with tumorigenicity and androgen receptor status of the cells. Mst4 kinase activity was stimulated significantly by epidermal growth factor receptor ligands, which are known to promote growth of prostate cancer cells (3).


1. Lin JL, et al. Oncogene 20(45):6559-69, 2001
2. Dan I, et al. J Biol Chem 277(8):5929-39, 2002
3. Sung V, et al. Cancer Res 63(12):3356-63, 2003

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Cat# AJ1605a
(40 western blots)
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