|Calculated MW||148532 Da|
|Other Names||1-phosphatidylinositol 4, 5-bisphosphate phosphodiesterase gamma-1, PLC-148, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-II, PLC-II, Phospholipase C-gamma-1, PLC-gamma-1, PLCG1, PLC1|
|Target/Specificity||A synthetic peptide corresponding to phosphorylated residue surrounding tyrosine 1245 of human PLCγI was used.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Phospho-PLC Antibody gamma I is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.|
|Cellular Location||Cell projection, lamellipodium. Cell projection, ruffle. Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment|
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Provided below are standard protocols that you may find useful for product applications.
The phospholipase C gamma-1 (PLCγI) is a member of phospholipase C family which catalyzes the hydrolysis of phospholipids to yield second messengers such as diacylglycerols (DAG) and water-soluble phosphorylated derivatives of the lipid head groups. These second messengers play a crucial role in transmembrane signaling and are involved in the regulation of several cellular functions, such as cell differentiation and proliferation (1). PLCγI interacts with variety of signaling molecules via its two SH2 domains and a single SH3 domain and it is activated by phosphorylation on Tyr783 through interaction with non-receptor and receptor tyrosine kinases. Interaction with activated receptors (e.g. PDGFR and EGFR) leads to phosphorylation on Tyr771, Tyr783, and Tyr1245 (2). Phosphorylations at Tyr783 and 1245 might be significant for adhesion PLCγI to fibronectin during integrin initiated fibronectin adhesion (3)
1. Kay EP et al. Mol Vis. 4:22, 1998
2. Kim, HK et al. Cell 65, 435-442, 1991
3. Tvorogov D et al. JCS 118:601-610, 2005
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