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>   home   >   Products   >   Primary Antibodies   >   PIAS1 Antibody (C-term)   

PIAS1 Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - PIAS1 Antibody (C-term) AJ1609a
    A. Western blot analysis on (A) Daudi (B) 293 (C) Jurkat cell lysate, dilution 1:50,000 and (D) SW480 and (E) HepG2 using anti-PIAS1 RabMAb (Cat. #AJ1609a)
  • IHC - PIAS1 Antibody (C-term) AJ1609a
    B. Immunohistochemical analysis of paraffin-embedded human tonsils using anti-PIAS1 RabMAb (Cat. #AJ1609a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O75925
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EPR2581Y
Calculated MW 71836 Da
Gene ID 8554
Other Names E3 SUMO-protein ligase PIAS1, 632-, DEAD/H box-binding protein 1, Gu-binding protein, GBP, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein, PIAS1, DDXBP1
Target/Specificity A synthetic peptide corresponding to residues on the C-terminus of human PIAS1 was used as an immunogen.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIAS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIAS1
Synonyms DDXBP1
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation.
Cellular Location Nucleus speckle. Nucleus, PML body Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton
Tissue Location Expressed in numerous tissues with highest level in testis.
Citations (0)

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PIAS1 (protein inhibitor of activated STAT) proteins blocks the DNA binding activity of Stat1 and inhibits Stat1-mediated gene activation in response to cytokine stimulation (1). PIAS1 also plays a crucial role as a transcriptional coregulator in the p53 pathway and the steroid hormone signaling pathway and functions as an E3 type small ubiquitin-like modifier (SUMO) ligas, stabilizing the interaction between UBEZ1 and the substrate, and as a SUMO-tethering factor. It is expressed in numerous tissues with highest level in testes.


1. Liu B, et al. Proc Natl Acad Sci 95(18):10626-31, 1998

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Cat# AJ1609a
(40 western blots)
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