- Antibodies
- New
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Tag
- Peptides
- Biological Process >
- Cellular Compartment >
- Disease >
- Molecular Function >
- Pathway Biocarta >
- Pathway KEGG >
- Pathway Panther >
- Tissue >
- Amino Acids
- Tag
- Biological Process >
- Proteins
- RNAi
- FL cDNA Clones
- Cell/Tissues/Lysates
PKA, RII Antibody (C-term)Rabbit Monoclonal Antibody
| Country | United States
Ordering Information
Australia Austria Belgium Brazil Bulgaria Canada China Croatia Cyprus Czech Republic Denmark Estonia Finland France Germany Greece Hungary Iceland India Indonesia Ireland Israel Italy Japan Korea Latvia Lithuania Luxembourg Macedonia Malaysia Malta Netherlands Norway Pakistan Poland Portugal Romania Serbia Singapore Slovakia Slovenia Spain Sweden Switzerland Taiwan Turkey United Kingdom United States Vietnam Others 
|
|||
|---|---|---|---|---|
| Catalog # | Size | Availability | Price | |
| AJ1614a | 100ul 400 ul | 2-3 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
PKA, RII Antibody (C-term) - Product info | |
| Application | WB, IHC, IF
|
| Primary Accession | P13861 |
| Reactivity | Human, Mouse |
| Clone Names | Y116 |
| Calculated MW | 45518 Da |
| Gene ID 5576 | |
| Other Names PRKAR2A, PKR2, PRKAR2, cAMP-dependent protein kinase type II-alpha regulatory subunit | |
| Target/Specificity A synthetic peptide corresponding to residues in the C-terminus of human cAMP-dependent protein kinase type II-alpha regulatory subunit was used as immunogen. The antibody does not cross-react with other cAMP-dependent kinase. | |
| Dilution WB~~1:500~1000 IHC~~1:100~250 | |
| Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions PKA, RII Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
PKA, RII Antibody (C-term) - Protein Information | |
| Name PRKAR2A | |
| Synonyms PKR2, PRKAR2 | |
| Function Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase | |
| Cellular Location Cytoplasm. Cell membrane. Note=Co-localizes with PJA2 in the cytoplasm and the cell membrane | |
| Tissue Location Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible | |
PKA, RII Antibody (C-term) - Related products
AP6823a: PRKAR2A Antibody (N-term)
AP6823c: PRKAR2A Antibody (Center)
AP8096a: PKA R2 (PKR2) Antibody (N-term)
RI14218: PRKAR2A predesign siRNA
LY10107a: PRKAR2A Over-expression Lysate
BP6823a: PRKAR2A Antibody (N-term) Blocking Peptide
BP6823c: PRKAR2A Antibody (Center) Blocking Peptide
BP8096a: PKR2 Antibody (N-term) Blocking Peptide
AT3430a: PRKAR2A Antibody (monoclonal) (M01)
PKA, RII Antibody (C-term) - Application data
-
A. Western blot analysis on K562 cell lysate using anti- PKA RII (C-term) RabMAb (Cat. #AJ1614a), dilution 50,000
-
B. Immunohistochemical analysis of paraffin-embedded human normal breast tissue using anti-PKA RII (C-term) RabMAb (Cat. #AJ1614a).
-
C. Immunofluorescent staining of HeLa cells using anti-PKA RII (C-term) RabMAb (Cat. #AJ1614a).
PKA, RII Antibody (C-term) - Research Areas
Abgent welcomes feedback from its customers.
If you have used an Abgent product and would like to share how it has performed, please click on the
"Submit Review" button and provide the requested information. Our staff will examine and post your
review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abgent.com.
Thank you for your support.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunits (RI and RII) have been identified, each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3).
REFERENCES
1. Montminy, M. Transcriptional regulation by cyclic AMP. Annu. Rev. Biochem. 66: 807
2. Beebe, S.J. The cAMP-dependent protein kinases and cAMP signal transduction. Semin Cancer Biol. 5: 285
3. Braun, R.K, et al. Phosphorylation of RII subunit and attenuation of cAMP-dependent protein kinase activity by proline-directed protein kinase. Arch Biochem Biophys. 289: 187
