Register or Login
  • All
  • Uniprot Id
  • Catalog #
  • Peptide Sequence
>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   Phospho Antibodies   >   PKA-C Antibody (gamma) Phospho-pT197   

PKA-C Antibody (gamma) Phospho-pT197

Rabbit Monoclonal Antibody

  • WB - PKA-C Antibody (gamma) Phospho-pT197 AJ1616a
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-PKA C-gamma (pT197) RabMAb (Cat. #AJ1616a), 1:5000 dilution. Cells were either (A) untreated (B) treated with calyculin A.
  • IHC - PKA-C Antibody (gamma) Phospho-pT197 AJ1616a
    B. Immunohistochemical analysis of paraffin-embedded human testis using anti-PKA RabMAb (Cat. #AJ1616a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22612
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP2606Y
Calculated MW 40434 Da
Gene ID 5568
Other Names cAMP-dependent protein kinase catalytic subunit gamma, PKA C-gamma, PRKACG
Target/Specificity A phospho specific peptide corresponding to residues surrounding threonine 197 of human PKA C-gamma was used as an immunogen. This antibody detects PKA C-gamma phosphorylated on thr197.
Dilution WB~~1:2500~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPKA-C Antibody (gamma) Phospho-pT197 is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
Tissue Location Testis specific. But important tissues such as brain and ovary have not been analyzed for the content of transcript
Research Areas
Citations (0)

Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.

Submit your citation using an Abgent antibody to, and receive a free "I Love Antibodies" mug.


PKA (or cAPK) is a cyclic AMP-dependent protein kinase. When activated by the second messenger cAMP, PKA mediates diverse cellular mechanisms, including proliferation, ion transport, regulation of metabolism, and gene transcription (1,2). PKA is comprised of two dimers of two subunits, R (regulatory) and C (catalytic). Two families of R subunit (RI and RII) and three C subunit isoforms (C-alpha, C-beta, and C-gamma) have been identified each possessing distinct cAMP binding properties and resulting in different phosphorylation states (2,3). C subunit is activated through autophosphorylation and direct phosphorylation at Thr197 by PDK-1. Tissue-specific expression of C gamma, suggests a pressure on C gamma during evolution, acting to modulate it in a functionally specific way. Certain amino acid substitutions make C gamma a distinct member of the cAMP-dependent subfamily of protein kinases, and suggest that C gamma may be distinct in its protein substrate specificity or its interaction with the different regulatory subunits (4).


1. Montminy, M, Annu. Rev. Biochem. 66: 807-822 (1997).
2. Beebe, SJ et al. Semin Cancer Biol. 5: 285-94 (1994).
3. Braun RK et al. Arch Biochem Biophys. 289: 187-91 (1991).
4. Beebe SJ et al. Mol Endocrinol 4(3):465-75, 1990

Abgent welcomes feedback from its customers.

If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.

If you have any additional inquiries please email technical services at

Cat# AJ1616a
(40 western blots)
Alternative Products:

Ordering Information

United States
AlbaniaAustraliaAustriaBelgiumBosnia & HerzegovinaBrazilBulgariaCanadaChinaCroatiaCyprusCzech RepublicDenmarkEstoniaFinlandFranceGermanyGreeceHong KongHungaryIcelandIndiaIndonesiaIrelandIsraelItalyJapanKoreaLatviaLithuaniaLuxembourgMacedoniaMalaysiaMaltaNetherlandsNew ZealandNorwayPakistanPolandPortugalRomaniaSerbiaSingaporeSlovakiaSloveniaSouth AfricaSpainSwedenSwitzerlandTaiwanTurkeyUnited KingdomUnited StatesVietnamOthers
Abgent, Inc.
(888) 735-7227 / (858) 622-0099
(858) 622-0609

Shipping Information

Domestic orders (in stock items)
Shipped out the same day. Orders placed after 1 PM (PST) will ship out the next business day.
International orders
Contact your local distributors
Santa Cruz Alternative
Terms & Conditions