|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||531791 Da|
|Other Names||Plectin, PCN, PLTN, Hemidesmosomal protein 1, HD1, Plectin-1, PLEC, PLEC1|
|Target/Specificity||A recombinant fragment comprising the C-terminal 220 amino acids of plectin was used as immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Plectin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Interlinks intermediate filaments with microtubules and microfilaments and anchors intermediate filaments to desmosomes or hemidesmosomes. Could also bind muscle proteins such as actin to membrane complexes in muscle. May be involved not only in the filaments network, but also in the regulation of their dynamics. Structural component of muscle. Isoform 9 plays a major role in the maintenance of myofiber integrity.|
|Cellular Location||Cytoplasm, cytoskeleton. Cell junction, hemidesmosome|
|Tissue Location||Widely expressed with highest levels in muscle, heart, placenta and spinal cord|
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Provided below are standard protocols that you may find useful for product applications.
Plectin, a 500-kDa intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).
1. Liu, C.G., et al. Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24). Proc Natl Acad Sci U S A. 93: 4278
2. Wiche, G. Role of plectin in cytoskeleton organization and dynamics. J. Cell. Sci. 111: 2477
3. Ruhrberg, C. and F.M. Watt. The plakin family: versatile organizers of cytoskeletal architecture. Curr. Opin. Genet. Dev. 7: 392
4. Foisner, R., et al. Protein kinase A and protein kinase C-regulated interaction of plectin with lamin B and vimentin. Proc. Natl. Acad. Sci. USA 88: 3812-3816 (1991).
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