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Pou Antibody (Domain Protein 3A/Brn-3a)

Rabbit Monoclonal Antibody

     
  • WB - Pou Antibody (Domain Protein 3A/Brn-3a) AJ1641a
    A. Western blot analysis on human brain lysate using anti-POU RabMAb (Cat. #AJ1641a).
  • IHC - Pou Antibody (Domain Protein 3A/Brn-3a) AJ1641a
    B. Immunohistochemical analysis of paraffin-embedded human placenta using anti-POU RabMAb (Cat. #AJ1641a).
  • IF - Pou Antibody (Domain Protein 3A/Brn-3a) AJ1641a
    C. Immunofluorescent staining of HeLa cells using anti-POU RabMAb (Cat. #AJ1641a).
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q01851
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1972Y
Calculated MW 42697 Da
Gene ID 5457
Other Names POU domain, class 4, transcription factor 1, Brain-specific homeobox/POU domain protein 3A, Brain-3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1, POU4F1, BRN3A, RDC1
Target/Specificity A synthetic peptide corresponding to residues near the C-terminus of human POU domain protein 3a was used as an immunogen.
Dilution WB~~1:10000
IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPou Antibody (Domain Protein 3A/Brn-3a) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name POU4F1 (HGNC:9218)
Function Multifunctional transcription factor with different regions mediating its different effects. Acts by binding through its C-terminal domain to sequences related to the consensus octamer motif 5'-ATGCAAAT-3' in the regulatory regions of their target genes. Regulates the expression of specific genes involved in differentiation and survival within a subset of neuronal lineages. It has been shown that some of these genes require its N-terminal in order to be activated, maybe through a neuronal- specific cofactor. Ativates BCL2 expression and protect neuronal cells from apoptosis, through the N-terminal domain. Induces neuronal process outgrowth and the coordinate expression of genes encoding synaptic proteins. Exerts its major developmental effects in somatosensory neurons and in brainstem nuclei involved in motor control. May positively regulate POU4F2 and POU4F3. Regulates dorsal root ganglion sensory neuron specification and axonal projection into the spinal cord. Negatively regulates its own expression interacting directly with a highly conserved autoregulatory domain surrounding the transcription initiation site.
Cellular Location Nucleus.
Tissue Location Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye
Research Areas
Citations (0)

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Background

Brn-3a is a transcription factor belonging to the class IV of POU domain transcription factors. It is expressed throughout the peripheral nervous system but especially in postmitotic sensory neurons of dorsal root ganglia. Brn-3a is known to regulate different genes involved in neuronal differentiation and survival (1). Characterization of Brn-3a suggests potential roles of Brn-3a in the development of retinal ganglion cells and sensory neurons, as well as potential roles in the pituitary gland and the immune system. Brn-3a is expressed in the pituitary gland and in a corticotroph cell line (2). The differentiation of the ND7 neuronal cell line to a nondividing phenotype bearing numerous neurite processes is accompanied by a dramatic increase in the levels of the activating POU family transcription factor Brn-3a and a corresponding fall in the levels of the closely related inhibitory factor Brn-3b. Overexpression of Brn-3a in these cells can induce neurite outgrowth and the activation of genes encoding synaptic vesicle proteins in the absence of a differentiation-inducing stimulus (3).

References

1. Calissano M, et al. Oncogene 22(35):5408-14, 2003
2. Gerrero MR, et al. Proc Natl Acad Sci 15(90):10841-5, 1993
3. Smith MD, et al. J Biol Chem 272(2):1382-8, 1997

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Discontinued
Cat# AJ1641a
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