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PP2A Antibody (N-term)

Rabbit Monoclonal Antibody

  • WB - PP2A Antibody (N-term) AJ1644b
    A. Western blot analysis on A: A431 cell lysate B: NIH 3T3 cell lysate using anti-PP2A (N-term) RabMAb (Cat. #AJ1644b), dilution 1:500.
  • IHC - PP2A Antibody (N-term) AJ1644b
    B. Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-PP2A (N-term) RabMAb (Cat. #AJ1644b).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P67775
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names YE351
Calculated MW 35594 Da
Gene ID 5515
Other Names Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, Replication protein C, RP-C, PPP2CA
Target/Specificity A synthetic peptide corresponding to residues in N-terminus of human PP2A-alpha was used as immunogen. Predicted to cross-react with bovin and pig, based on sequence homology.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPP2A Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Function PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Cellular Location Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity).
Research Areas
Citations (0)

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Protein phosphatase type 2A (PP2A) is a protein serine/threonine phosphatase that controls fundamental cellular processes, including transcription, translation, metabolism, cell growth, apoptosis, and varied other signal transduction pathways (1-3). PP2A is comprised of a core enzyme, which is made up of catalytic C and regulatory A (PR65) subunits, and a secondary regulatory B subunit. This B subunit contains four different protein families. In response to EGF or insulin, it has been shown that Src phosphorylates PP2A at Tyr307, thereby inactivating PP2A (4).


1. Janssens, V. and J. Goris. Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling. Biochem. J. 353: 417
2. Zabrocki, P., et al. Protein phosphatase 2A on track for nutrient-induced signalling in yeast. Mol. Microbiol. 43: 835
3. Milward, T.A., et al. Regulation of protein kinase cascades by protein phosphatase 2A. Trends Biochem. Sci. 24: 186
4. Chen, J., et al. Regulation of protein serine-threonine phosphatase type-2A by tyrosine phosphorylation. Science 257: 1261

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Cat# AJ1644b
(40 western blots)
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