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PP2C alpha Antibody

Rabbit Monoclonal Antibody

  • WB - PP2C alpha Antibody AJ1646a
    A. Western blot analysis on SH-SY5Y cell lysate using anti-PP2C-alpha RabMAb (Cat. #AJ1646a), 1:2000 dilution.
  • IHC - PP2C alpha Antibody AJ1646a
    B. Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using anti-PP2C-alpha RabMAb (Cat. #AJ1646a).
  • IHC - PP2C alpha Antibody AJ1646a
    C. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PP2C-alpha RabMAb (Cat. #AJ1646a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P35813
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names EP1684Y
Calculated MW 42448 Da
Gene ID 5494
Other Names Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, PP2C-alpha, Protein phosphatase IA, PPM1A, PPPM1A
Target/Specificity A synthetic peptide corresponding to residues near the C-terminal of human PP2C alpha protein was used as an immunogen.
Dilution WB~~1:10000~50000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPP2C alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PPM1A
Synonyms PPPM1A
Function Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.
Cellular Location Nucleus. Cytoplasm, cytosol. Membrane. Note=Weakly associates at the membrane and N- myristoylation mediates the membrane localization.
Research Areas
Citations (0)

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Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C-alpha functions by dephosphorylating various substrates (e.g. Smad1, MAPK) and it is considered to be a negative regulator of cell stress pathway (2). PP2C-alpha has been implicated in the regulation of apoptosis-, p53-, cyclin-dependent kinase-, and TGF beta- signaling. Studies have suggested tumor-suppressing properties of PP2C -alpha and its possible role in cancer development and progression (3).


1. Baudouin E., et al. Plant Journal, 20(3):343-348, 1999.
2. Takekawa, M., et al, EMBO J. 17: 4744-4752, 1998.
3. Lammers T, et al. Molecular Cancer. 6:65, 2007.

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Cat# AJ1646a
(40 western blots)
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