|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||42448 Da|
|Other Names||Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, PP2C-alpha, Protein phosphatase IA, PPM1A, PPPM1A|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminal of human PP2C alpha protein was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PP2C alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Enzyme with a broad specificity. Negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3, resulting in their dissociation from SMAD4, nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. Dephosphorylates PRKAA1 and PRKAA2. Plays an important role in the termination of TNF-alpha-mediated NF-kappa-B activation through dephosphorylating and inactivating IKBKB/IKKB.|
|Cellular Location||Nucleus. Cytoplasm, cytosol. Membrane. Note=Weakly associates at the membrane and N- myristoylation mediates the membrane localization.|
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Provided below are standard protocols that you may find useful for product applications.
Protein phosphatase 2C (PP2C) is a family of serine/threonine specific protein phosphatases. PP2C is a 42 kDa monomeric enzyme with broad substrate specificity and its activity is depended upon divalent cations (e.g. magnesium) (1). In mammals, three members of PP2C family are known; PP2C -alpha, -beta, and -gamma. PP2C-alpha functions by dephosphorylating various substrates (e.g. Smad1, MAPK) and it is considered to be a negative regulator of cell stress pathway (2). PP2C-alpha has been implicated in the regulation of apoptosis-, p53-, cyclin-dependent kinase-, and TGF beta- signaling. Studies have suggested tumor-suppressing properties of PP2C -alpha and its possible role in cancer development and progression (3).
1. Baudouin E., et al. Plant Journal, 20(3):343-348, 1999.
2. Takekawa, M., et al, EMBO J. 17: 4744-4752, 1998.
3. Lammers T, et al. Molecular Cancer. 6:65, 2007.
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