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PRC1 Antibody Phospho (pT481)

Rabbit Monoclonal Antibody

     
  • WB - PRC1 Antibody Phospho (pT481) AJ1648b
    A. Western blot analysis on HeLa cell lysates using anti-Phospho-PRC1 (pT481) RabMAb (Cat. #AJ1648b), 1:500 dilution. Cells were either (A) untreated (B) treated with aphidicolin + nocodazole.
  • IF - PRC1 Antibody Phospho (pT481) AJ1648b
    B. Immunofluorescent staining of HeLa cells using anti-PRC1 RabMAb (Cat. #AJ1648b).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession O43663
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1514Y
Calculated MW 71607 Da
Gene ID 9055
Other Names Protein regulator of cytokinesis 1, PRC1 {ECO:0000312|EMBL:AAC026881}
Target/Specificity A phospho specific peptide corresponding to residues surrounding Threonine 481 of human PRC1 was used as an immunogen. This antibody detects PRC1 phosphorylated at Theonine 481.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRC1 Antibody Phospho (pT481) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PRC1 (HGNC:9341)
Function Key regulator of cytokinesis that cross-links antiparrallel microtubules at an average distance of 35 nM. Essential for controlling the spatiotemporal formation of the midzone and successful cytokinesis. Required for KIF14 localization to the central spindle and midbody. Required to recruit PLK1 to the spindle. Stimulates PLK1 phosphorylation of RACGAP1 to allow recruitment of ECT2 to the central spindle. Acts as an oncogene for promoting bladder cancer cells proliferation, apoptosis inhibition and carcinogenic progression (PubMed:17409436).
Cellular Location Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Midbody. Note=Colocalized with KIF20B in the nucleus of bladder carcinoma cells at the interphase. Colocalized with KIF20B in bladder carcinoma cells at prophase, metaphase, early anaphase, at the midzone in late anaphase and at the contractile ring in telophase (PubMed:17409436). Predominantly localized to the nucleus of interphase cells. During mitosis becomes associated with the mitotic spindle poles and localizes with the cell midbody during cytokinesis
Tissue Location Overexpressed in bladder cancer cells (PubMed:17409436).
Research Areas
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Background

Novel human protein Protein regulator of cytokinesis 1 (PRC1) is involved in cytokinesis. PRC1 is a good substrate for several CDKs in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, strongly suggesting that PRC1 is an in vivo CDK substrate. PRC1 protein levels are high during S and G2/M and drop dramatically after cells exit mitosis and enter G1. PRC1 is a nuclear protein in interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis (1). PRC1, a mitotic spindle-associated Cdk substrate that is essential to cell cleavage, is a microtubule binding and bundling protein both in vivo and in vitro. Overexpression of PRC1 extensively bundles interphase microtubules, but does not affect early mitotic spindle organization. PRC1 is phosphorylated by Cdc2 at Thr470 and Thr481 during mitosis. (2). PRC1 has been shown to down-regulate the GAP activity of MgcRac-GAP during the metaphase and thereby contributes to the correct formation of the spindle (3).

References

1. Jiang W, et al. Mol Cell 2(6):877-85, 1998.
2. Mollinari C, et al. J. Cell Biol 157(7):1175-86, 2002
3. Ban R, et al. J Biol Chem 279(16):16394-402, 2004.

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Discontinued
Cat# AJ1648b
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