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PRK2 Antibody

Rabbit Monoclonal Antibody

  • WB - PRK2 Antibody AJ1653a
    A. Western blot analysis on 293T cell lysate using anti-PRK2 RabMAb (Cat. #AJ1653a), dilution 1:1,000.
  • IHC - PRK2 Antibody AJ1653a
    B. Immunohistochemical analysis of paraffin-embedded human colon carcinoma using anti-PRK2 RabMAb (Cat. #AJ1653a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q16513
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names E352
Calculated MW 112035 Da
Gene ID 5586
Other Names Serine/threonine-protein kinase N2, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2, PKN2, PRK2, PRKCL2
Target/Specificity A synthetic peptide corresponding to residues near N-terminus of human PRK2 was used as immunogen.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPRK2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PKN2
Synonyms PRK2, PRKCL2
Function PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c- fos serum response factor (SRF). Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.
Cellular Location Cytoplasm. Nucleus. Membrane. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction. Note=Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm
Tissue Location Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells
Research Areas
Citations (0)

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Protein-kinase-C-related kinases (PRKs) (1) are part of the lipid-regulated protein kinases (PKC) which also include liver PAK & PKN (2-3). Human PRK1 and PRK2 share structurally similar catalytic domains, but less similar N-terminal regulatory regions suggesting different regulatory domain functions. PRK1 and PRK2, as well as a third member of this family, PRK3, show distinct patterns of expression in adult tissues. Additionally, the serine-threonine kinase PRK2 can be specifically cleaved by caspase-3 (and/or caspase-3-like subfamily members) during apoptosis (4).


1. Nishizuka, Y (1992) Science 258, 607-614
2. Gabrielli, B., Wettenhall, R. E. H., Kemp, B. E., Quinn, M., and Bozinova, L. (1984) FEBS Lett. 175, 219-226
3. Mukai, H., and Ono, Y. (1994) Biochem. Biophys. Res. Commun. 199, 897-904
4. Palmer, R. H., and Parker, P. J. (1995) Eur. J. Biochem. 227, 344-351

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Cat# AJ1653a
(40 western blots)
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