|Application ||WB, IHC|
|Calculated MW||98981 Da|
|Other Names||Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3|
|Target/Specificity||A synthetic peptide corresponding to residues near N-terminus of human Progesterone Receptor was used as immunogen. The antibody does not cross-react with other NR3 family members.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Progesterone Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.|
|Cellular Location||Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases Isoform 4: Mitochondrion outer membrane|
|Tissue Location||In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region- specific responses to hormonal stimuli|
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Provided below are standard protocols that you may find useful for product applications.
The human progesterone receptor (PR), is a ligand-activated transcription factor and is a member of the steroid receptor family (1). PR exists in humans as two isoforms; PR-A (94 kDa) which lacks the first 164 amino acids of PR-B and PR-B (114 kDa)(2-3). While the two forms of PR have similar DNA- and ligand-binding affinities they have opposite transcriptional activities. PR-B functions as an activator of progesterone-responsive genes, while PR-A functions as a strong transdominant repressor of PR-B (4)
1. Evans, R. M. 1988. The steroid and thyroid hormone receptor superfamily. Science 240:889-895
2. Paloma H. Giangrande, Erin A. Kimbrel Dean P. Edwards and Donald P. McDonnell, Molecular and Cellular Biology, May 2000, p. 3102-3115, Vol. 20, No. 9
3. Giangrande, P. H., G. Pollio, and D. P. McDonnell. 1997. Mapping and characterization of the functional domains responsible for the differential activity of the A and B isoforms of the human progesterone receptor. J. Biol. Chem. 272:32889-32900
4. B. Mulac-Jericevic and O. M Conneely. Reproductive tissue selective actions of progesterone receptors. Reproduction, August 1, 2004; 128(2): 139
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