|Application ||WB, IHC|
|Calculated MW||98981 Da|
|Other Names||Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding serine 190 of human PR was used as an immunogen. This antibody detects PR phophorylated at serine 190.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Progesterone Antibody Phospho (pS190) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. Isoform 4: Increases mitochondrial membrane potential and cellular respiration upon stimulation by progesterone.|
|Cellular Location||Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases Isoform 4: Mitochondrion outer membrane|
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Provided below are standard protocols that you may find useful for product applications.
The human progesterone receptor (PR), is a ligand-activated transcription factor and is a member of the steroid receptor family (1). PR exists in humans as two isoforms; PR-A (94 kDa) which lacks the first 164 amino acids of PR-B and PR-B (114 kDa)(2-3). While the two forms of PR have similar DNA- and ligand-binding affinities they have opposite transcriptional activities. PR-B functions as an activator of progesterone-responsive genes, while PR-A functions as a strong transdominant repressor of PR-B (4). Human progesterone receptor (PR) is phosphorylated on multiple serine residues (at least seven sites) in a manner that involves distinct groups of sites coordinately regulated by hormone and different kinases. As detected by Western blot of T47D cells treated with hormone for different times, hormone-dependent down-regulation of total PR and the Ser 190 phosphorylation site occurred in parallel, whereas the Ser 294 phosphorylation site was down-regulated more rapidly (5)
1. Evans, R. M. 1988. Science 240:889-895
2. Paloma H. et al. May 2000, p. 3102-3115, Vol. 20, No. 9
3. Giangrande, P. et al. 1997. J. Biol. Chem. 272: 32889-32900
4. B. Mulac-Jericevic et al. Reproduction, 2004; 128(2): 139
5. Clemm DL, et al. Mol Edocrinol 14(1):52-65, 2000
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