|Application ||WB, IHC|
|Calculated MW||98981 Da|
|Other Names||Progesterone receptor, PR, Nuclear receptor subfamily 3 group C member 3, PGR, NR3C3|
|Target/Specificity||A phospho specific peptide corresponding to residues surrounding serine 190 of human PR was used as an immunogen. This antibody detects PR phophorylated at serine 190.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Progesterone Antibody Phospho (pS190) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Depending on the isoform, progesterone receptor functions as transcriptional activator or repressor.|
|Cellular Location||Nucleus. Cytoplasm. Note=Nucleoplasmic shuttling is both homone- and cell cycle-dependent. On hormone stimulation, retained in the cytoplasm in the G(1) and G(2)/M phases Isoform 4: Mitochondrion outer membrane|
|Tissue Location||In reproductive tissues the expression of isoform A and isoform B varies as a consequence of developmental and hormonal status. Isoform A and isoform B are expressed in comparable levels in uterine glandular epithelium during the proliferative phase of the menstrual cycle. Expression of isoform B but not of isoform A persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Heterogeneous isoform expression between the glands of the endometrium basalis and functionalis is implying region- specific responses to hormonal stimuli|
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Provided below are standard protocols that you may find useful for product applications.
The human progesterone receptor (PR), is a ligand-activated transcription factor and is a member of the steroid receptor family (1). PR exists in humans as two isoforms; PR-A (94 kDa) which lacks the first 164 amino acids of PR-B and PR-B (114 kDa)(2-3). While the two forms of PR have similar DNA- and ligand-binding affinities they have opposite transcriptional activities. PR-B functions as an activator of progesterone-responsive genes, while PR-A functions as a strong transdominant repressor of PR-B (4). Human progesterone receptor (PR) is phosphorylated on multiple serine residues (at least seven sites) in a manner that involves distinct groups of sites coordinately regulated by hormone and different kinases. As detected by Western blot of T47D cells treated with hormone for different times, hormone-dependent down-regulation of total PR and the Ser 190 phosphorylation site occurred in parallel, whereas the Ser 294 phosphorylation site was down-regulated more rapidly (5)
1. Evans, R. M. 1988. Science 240:889-895
2. Paloma H. et al. May 2000, p. 3102-3115, Vol. 20, No. 9
3. Giangrande, P. et al. 1997. J. Biol. Chem. 272: 32889-32900
4. B. Mulac-Jericevic et al. Reproduction, 2004; 128(2): 139
5. Clemm DL, et al. Mol Edocrinol 14(1):52-65, 2000
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