|Application ||WB, IHC, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||29804 Da|
|Other Names||Prohibitin, PHB|
|Target/Specificity||A synthetic peptide corresponding to residues in human prohibitin was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Prohibitin Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.|
|Cellular Location||Mitochondrion inner membrane.|
|Tissue Location||Widely expressed in different tissues.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Prohibitin (PHB) proteins have been ascribed various functions, including cell cycle regulation, apoptosis, assembly of mitochondrial respiratory chain enzymes, and aging. It has been shown that the mammalian prohibitins are present in the inner mitochondrial membrane and are always bound to each other, with no free protein detectable. They are coexpressed during development and in adult mammalian tissues, and expression levels are indicative of a role in mitochondrial metabolism, but are not compatible with roles in the regulation of cellular proliferation or apoptosis. Data support conserved roles for the prohibitins in regulating mitochondrial respiratory activity and in aging (1). Results suggest that prohibitin may be a tumor suppressor and is associated with tumor development and/or progression of at least some breast cancers (2).
1. Coates PJ, et al. Exp. Cell Res. 265(2):262-73, 2001
2. Sato T, et al. Cancer Res. 52(6):1643-6, 1992
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.