|Application ||WB, IF|
|Calculated MW||84331 Da|
|Other Names||Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase, FOLH1, FOLH, NAALAD1, PSM, PSMA|
|Target/Specificity||A synthetic peptide corresponding to residues near the C-terminus of human PSMA was used as an immunogen.|
|Format||50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PSMA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FOLH, NAALAD1, PSM, PSMA|
|Function||Has both folate hydrolase and N-acetylated-alpha-linked- acidic dipeptidase (NAALADase) activity. Has a preference for tri- alpha-glutamate peptides. In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N- aceylaspartylglutamate (NAAG), thereby releasing glutamate. Involved in prostate tumor progression.|
|Cellular Location||Cell membrane; Single-pass type II membrane protein|
|Tissue Location||Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-3 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer|
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PSMA (Prostate-specific membrane antigen, Glutamate carboxypeptidase 2) is a membrane peptidase expressed in the prostate, central and peripheral nervous system, kidney, and small intestine (1). PSMA has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. It is required in the intestine for the uptake of folate, and in the brain it modulates excitatory neurotransmission through the hydrolysis of the neuropeptide N-acetylaspartylglutamate, thereby releasing glutamate (2). In the central nervous system, it is responsible for the cleavage of N-acetyl- L-aspartyl-L-glutamate yielding free glutamate in the synaptic cleft, and is implicated in various pathologic conditions associated with glutamate excitotoxicity (1). PSMA also has abundant and restricted expression on the surface of prostate carcinomas and the neovasculature of most other solid tumors, making it a target for cancer immunotherapy (3).
1. Barinka C, Protein Science, 13:1627-1635, 2004
2. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008)
3. Schulke N, PNAS 100(22):12590-12595, 2003
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