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PTEN Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - PTEN Antibody (C-term) AJ1664a
    A. Western blot analysis on MCF7 cell lysate using anti-PTEN (C-term) RabMAb (Cat. #AJ1664a), dilution 1:500
  • IHC - PTEN Antibody (C-term) AJ1664a
    B. Immunohistochemical analysis of paraffin-embedded human thyroid gland carcinoma using anti-PTEN (C-term) RabMAb (Cat. #AJ1664a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P60484
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Monoclonal
Clone Names Y184
Calculated MW 47166 Da
Gene ID 5728
Other Names Phosphatidylinositol 3, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN, MMAC1, TEP1
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human PTEN was used as immunogen.
Dilution WB~~1:500
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTEN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms MMAC1, TEP1
Function Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement.
Cellular Location Cytoplasm. Nucleus. Nucleus, PML body. Note=Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies XIAP/BIRC4 promotes its nuclear localization
Tissue Location Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas.
Research Areas
Citations (0)

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PTEN is a protein tyrosine phosphatase that acts as a tumor suppressor, as a lipid phosphatase that dephosphorylates the D3 position of phosphatidylinositol 3,4,5-trisphosphate and as an antagonist of the PI3k/AKT signaling pathway (1-3). PTEN structural domains includes an N-terminal phosphatase domain, a lipid binding C2 domain and a 50-amino acid C-terminal tail that contains a PD biding sequence (5). Phosphorylation of the tail of the protein will suppress its activity and modify its conformation to a closed form an open form (5). Mutations in PTEN have been linked to glioblastoma, melanoma and prostate cancer.


1. Vazquez, F., and Sellers, W. R. (2000) Biochim. Biophys. Acta 1470, M21-M35
2. Maehama, T., and Dixon, J. E. (1998) J. Biol. Chem. 273, 13375-13378
3. Maehama, T., Taylor, G. S., and Dixon, J. E. (2001) Annu. Rev. Biochem. 70, 247-279
4. Songyang, Z., Fanning, A. S., Fu, C., Xu, J., Marfatia, S. M., Chishti, A. H., Crompton, A., Chan, A. C., Anderson, J. M. & Cantley, L. C. (1997) Science 275, 73-77
5. Francisca Vazquez, Steven R. Grossman, Yuki Takahashi, Mihail V. Rokas, Noriaki Nakamura, and William R. Sellers, J. Biol. Chem., Vol. 276, Issue 52, 48627-48630, December 28, 2001

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Cat# AJ1664a
(40 western blots)
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