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PTP1B Antibody Phospho (pS50)

Rabbit Monoclonal Antibody

  • WB - PTP1B Antibody Phospho (pS50) AJ1665e
    A. Western blot analysis on Hela cell lysates using anti-Phospho-PTP1B (pS50) RabMAb (Cat. #AJ1665e), 1:5000 dilution. Cells were either (A) untreated (B) treated with Calyculin A.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P18031
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP1838Y
Calculated MW 49967 Da
Gene ID 5770
Other Names Tyrosine-protein phosphatase non-receptor type 1, Protein-tyrosine phosphatase 1B, PTP-1B, PTPN1, PTP1B
Target/Specificity A phospho specific peptide corresponding to residues surround Serine 50 of human PTP1B was used as an immunogen. This antibody detects PTP1B phosphorylated at Serine 50.
Dilution WB~~1:1000~10000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTP1B Antibody Phospho (pS50) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTPN1
Synonyms PTP1B
Function Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side Note=Interacts with EPHA3 at the cell membrane
Research Areas
Citations (0)

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The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).


1. Flint AJ, et al. EMBO J 12(5):1937-46, 1993
2. Frangioni JV, et al. Cell 68(3):545-60, 1992
3. Ravichandran LV, et al Mol Endocrinol 5(10):1768-80, 2001

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Cat# AJ1665e
(40 western blots)
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