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PUMA Antibody (C-term)

Rabbit Monoclonal Antibody

  • WB - PUMA Antibody (C-term) AJ1666a
    A. Western blot analysis on K562 cell lysate using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a), dilution 1:5,000.
  • IF - PUMA Antibody (C-term) AJ1666a
    B. Immunofluorescent staining of HeLa cells using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).
  • IHC - PUMA Antibody (C-term) AJ1666a
    C. Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).
  • IHC - PUMA Antibody (C-term) AJ1666a
    D. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-PUMA (C-term) RabMAb (Cat. #AJ1666a).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BXH1
Reactivity Human
Host Rabbit
Clonality Monoclonal
Clone Names EP512Y
Calculated MW 20532 Da
Gene ID 27113
Other Names Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis, BBC3, PUMA
Target/Specificity A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen. This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recognized on the western blot gel.
Dilution WB~~1:1000~5000
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPUMA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BBC3
Synonyms PUMA
Function Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.
Cellular Location Mitochondrion Note=Localized to the mitochondria in order to induce cytochrome c release
Tissue Location Ubiquitously expressed.
Research Areas
Citations ( 0 )


PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cells undergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA induces cytochrome c release and activates the rapid induction of programmed cell death. PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrial translocation and multimerization of BAX. PUMA also plays a role in mediating p53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Two isoform of PUMA are known; PUMA-alpha and PUMA-beta.


1. Yu, J., Zhang, L., Hwang, P.M., Kinzler, K.W. and Vogelstein, B., 2001. Mol. Cell 7, pp. 673
2. Nakano, K. and Vousden, K.H., 2001. Mol. Cell 7, pp. 683
3. Han, J. et al. 2001. Proc. Natl. Acad. Sci. USA 98, pp. 11318

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Cat# AJ1666a
(40 western blots)
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