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Pumilio-1 Antibody

Rabbit Monoclonal Antibody

     
  • WB - Pumilio-1 Antibody AJ1667a
    Western blot analysis on (A) HeLa (3 min. exposure) and (B) 293T cell lysates using anti-Pumilio-1 RabMAb (Cat. #AJ1667a).
  • IHC - Pumilio-1 Antibody AJ1667a
    Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue using anti-Pumilio 1 RabMAb (Cat. #AJ1667a).
  • IF - Pumilio-1 Antibody AJ1667a
    Immunofluorescent staining of HeLa cells using anti-Pumilio 1 RabMAb (Cat. #AJ1667a).
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q14671
Reactivity Human, Mouse
Host Rabbit
Clonality Monoclonal
Clone Names EPR3795
Calculated MW 126473 Da
Gene ID 9698
Other Names Pumilio homolog 1, HsPUM, Pumilio-1, PUM1, KIAA0099, PUMH1
Target/Specificity A synthetic peptide corresponding to residues in human Pumilio-1 was used as an immunogen. This antibody is predicted to not cross react with Pumilio-2.
Dilution WB~~1:10000~20000
IHC~~1:100~250
Format 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPumilio-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PUM1 (HGNC:14957)
Function Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE) (PubMed:21572425, PubMed:18328718, PubMed:21653694, PubMed:21397187). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation (PubMed:22955276). Also mediates deadenylation-independent repression by promoting accessibility of miRNAs (PubMed:18776931, PubMed:20818387, PubMed:20860814, PubMed:22345517). Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA- binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle (PubMed:20818387). Acts as a post-transcriptional repressor of E2F3 mRNAs by binding to its 3'-UTR and facilitating miRNA regulation (PubMed:22345517). Represses a program of genes necessary to maintain genomic stability such as key mitotic, DNA repair and DNA replication factors. Its ability to repress those target mRNAs is regulated by the lncRNA NORAD (non-coding RNA activated by DNA damage) which, due to its high abundance and multitude of PUMILIO binding sites, is able to sequester a significant fraction of PUM1 and PUM2 in the cytoplasm (PubMed:26724866). Involved in neuronal functions by regulating ATXN1 mRNA levels: acts by binding to the 3'-UTR of ATXN1 transcripts, leading to their down-regulation independently of the miRNA machinery (PubMed:25768905). Plays a role in cytoplasmic sensing of viral infection (PubMed:25340845). In testis, acts as a post-transcriptional regulator of spermatogenesis by binding to the 3'-UTR of mRNAs coding for regulators of p53/TP53. Involved in embryonic stem cell renewal by facilitating the exit from the ground state: acts by targeting mRNAs coding for naive pluripotency transcription factors and accelerates their down- regulation at the onset of differentiation (By similarity).
Cellular Location Cytoplasm. Cytoplasm, P-body. Cytoplasmic granule. Note=Recruited to cytoplasmic stress granules upon viral infection
Tissue Location Expressed in brain, heart, kidney, muscle, intestine and stomach. Not expressed in cerebellum, corpus callosum, caudate nucleus, hippocampus, medulla oblongata and putamen. Expressed in all fetal tissues tested
Research Areas
Citations (0)

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Background

Pumilio-1 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. It belongs to the PUF family, 84% identical to Pumilio-1. It is required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts (1). Pumilio-1 is highly expressed in testis and ovary and is predominately expressed in stem cells and germ cells.

References

1. The UniProt Consortium, The Universal Protein Resource (UniProt), Nucleic Acids Res. 36:D190-D195 (2008) 2. Wang X, et al. Mol Cell 7(4) :855-65, 2001

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Discontinued
Cat# AJ1667a
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